Gene/Protein
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Symptom
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Enzyme
Compound
Pivot Concepts:
Gene/Protein
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Target Concepts:
Gene/Protein
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Query: EC:2.7.11.2 (
PDK1
)
2,238
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Epinephrine
inhibits insulin release by activation of K(+) channels and subsequent hyperpolarization of pancreatic beta cells. The present study explored whether epinephrine-induced hyperpolarization is modified by phosphatidylinositol 3-kinase (PI3K) and phosphatidylinositide-dependent kinase
PDK1
. Perforated patch-clamp was performed in islet cells isolated from
PDK1
hypomorphic mice (pdk1(fl/fl)), expressing only 20% of
PDK1
, and in their wild-type littermates. At 16.8mM glucose, the cell membrane was hyperpolarized by epinephrine (1 microM), an effect significantly blunted in pdk1(fl/fl) and abrogated in wild-type cells by inhibition of PI3K with wortmannin (100 nM) or LY294002 (10 microM). The hyperpolarizing effect of epinephrine in pancreatic islet cells is thus sensitive to PI3K and
PDK1
.
...
PMID:Epinephrine-induced hyperpolarization of pancreatic islet cells is sensitive to PI3K-PDK1 signaling. 1971 69
