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Target Concepts:
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Query: EC:2.7.11.17 (
CaMKII
)
4,029
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Ca2+/calmodulin-dependent protein kinase I (CaMKI), originally identified as a protein kinase phosphorylating synapsin I, has been shown to constitute a family of closely related isoforms (alpha, beta and gamma). Here, we have isolated and determined the complete primary structures of two alternatively splicing isoforms of CaMKI termed
CaMKI gamma
1 and -gamma 2.
CaMKI gamma
1 and -gamma 2 contain an identical N-terminal catalytic domain with different C-terminal regions due to the deletion of the 425-bp nucleotide sequence of
CaMKI gamma
1 in
CaMKI gamma
2. In vitro kinase assay has demonstrated the marked enhancement of the Ca2+/CaM-dependent activity of
CaMKI gamma
1 by the preincubation with
Ca2+/calmodulin-dependent protein kinase kinase
(CaMKK), but no significant activation of
CaMKI gamma
2. Northern blot analysis has demonstrated the predominant expression of
CaMKI gamma
in the brain. RT-PCR analysis has revealed similar expression patterns between
CaMKI gamma
1 and
CaMKI gamma
2 in various brain regions. In situ hybridization analysis has demonstrated that
CaMKI gamma
mRNA is expressed in a distinct pattern from other isoforms of CaMKI with predominant expression in some restricted brain regions such as the olfactory bulb, hippocampal pyramidal cell layer of CA3, central amygdaloid nuclei, ventromedial hypothalamic nucleus and pineal gland. In the primary hippocampal neurons and NG108-15 cells, transfected
CaMKI gamma
1 and -gamma 2 are localized primarily in the cytoplasm and neurites but not in the nucleus. These findings suggest that both isoforms of
CaMKI gamma
may be involved in Ca2+ signal transduction in the cytoplasmic compartment of certain neuronal population.
...
PMID:Cloning, characterization and expression of two alternatively splicing isoforms of Ca2+/calmodulin-dependent protein kinase I gamma in the rat brain. 1275 81
