Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.17 (
CaMKII
)
4,029
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
5-Hydroxytryptamine (5-HT, 'serotonin') is a potent inducer of the early response gene cyclo-oxygenase 2 (
Cox-2
; prostaglandin G/H synthase) in mesangial cells. Protein kinase C (PKC), Ca2+-dependent enzymes and mitogen-activated protein kinase (p42/44 MAPK) have previously been shown to be essential modules of the signalling pathway leading from the pertussis-insensitive 5-HT2A receptor to the induction of
Cox-2
mRNA expression. In the present study, PKC activation was linked to the 5-HT-mediated phosphorylation and thus the activation of p42/44 MAPK: the inhibition of PKC by the specific inhibitor GF109203x prevented p42/44 MAPK activation. Ca2+/calmodulin-dependent (CaM) kinase II delta2 was detected in mesangial cells by Western blot analysis. The inhibition of
CaM kinase
by the inhibitors KN62 or KN93 led to a partial inhibition of 5-HT-induced
Cox-2
mRNA expression and decreased basal, but not PMA-mediated,
Cox-2
expression. The 5-HT-mediated activation of MAPK was not decreased by KN62 or KN93, excluding
CaM kinase
as a signalling module upstream of p42/44 MAPK. Taken together, these results indicate a modulatory involvement of
CaM kinase
in the regulation of 5-HT-mediated
Cox-2
mRNA expression in addition to the main pathway that consists of the activation of PKC and p42/44 MAPK.
...
PMID:Independent regulation of cyclo-oxygenase 2 expression by p42/44 mitogen-activated protein kinases and Ca2+/calmodulin-dependent kinase. 1019 Dec 63
