Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.17 (
CaMKII
)
4,029
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Secretory glands including salivary glands by many hormonal inputs produce and secrete biological fluids determined by variety of ion transporters. Spinophilin is a multifunctional scaffolding protein, which involved in receptor signaling and regulation of anion exchangers
AE2
activity. We found that spinophilin expressed in salivary glands. The role of salivary spinophilin on the modulation of chloride/bicarbonate exchange remains unknown. The spinophilin enhanced
AE2
activity and associated with a STE20/SPS1-related kinase and showed an additive effect on the modulation of the activity of
AE2
. The cholinergic stimulation and subsequent intracellular Ca
2+
increase was required for the interaction with
AE2
and spinophilin and abrogated the enhanced effect of spinophilin on Cl
-
transporting activity. Ductal chloride/bicarbonate exchange activity was increased in pretreatment with carbachol. The
CaMKII
inhibitor KN-93 suppressed the chloride/bicarbonate exchange activity of ducts, suggesting that
CaMKII
was required for ductal chloride/bicarbonate exchange activity. Additionally, microtubule destabilization by nocodazole attenuated the interaction of
AE2
and spinophilin and almost abolished the ductal chloride/bicarbonate exchange activity. The treatment of siRNA-spinophilin on the isolated salivary ducts also reduced the ductal chloride/bicarbonate exchange activity. Therefore, role of salivary spinophilin on
AE2
may facilitate the Cl
-
influx from basolateral in salivary glands in response to cholinergic inputs.
...
PMID:Chloride Influx of Anion Exchanger 2 Was Modulated by Calcium-Dependent Spinophilin in Submandibular Glands. 3007 10
