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Disease
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Enzyme
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Gene/Protein
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Target Concepts:
Gene/Protein
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Query: EC:2.7.11.17 (
CaMKII
)
4,029
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
This study reports the identification of splice variants for the calcium/calmodulin-dependent protein kinase II (
CaMKII
) gene from
Nilaparvata lugens
,
Laodelphax striatellus
, and
Sogatella furcifera
.
CaMKII
is a multifunctional serine/threonine protein kinase that transduces Ca
2+
signals in cells to control a range of cellular processes in the nervous system and muscular tissue. Sequence analysis showed that
CaMKII
was 99.0% identical at the amino acid level among three rice planthoppers, with the exception of a variable region located in the association domain. Four kinds of 20-81 amino acid "inserts" were found in the variable region. The phylogenetic tree of the deduced amino acid sequences showed that the NlCaMKII isoforms were more closely related to the LsCaMKII isoforms and were slightly distinct from SfCaMKII.
CaMKII
-E was the dominant type among the five main isoforms.
CaMKII
genes were constitutively expressed in various nymphal and adult stages and in tested tissues with the predominant transcription occurring in the head. There was no major tissue specificity of isoform expression, but the expression pattern and relative abundance of isoforms varied when compared with the RT-PCR between tissues. In addition, RNAi in
N. lugens
with dsRNA at a concentration of 200 ng nymph
-1
induced a mortality of 77.7% on the 10th day and a reduction in the mRNA expression level of 67.2%. Unlike the holometabolous insect
Helicoverpa armigera
, the knockdown of
NlCaMKII
did not suppress the expression of 20E response genes, such as
ECR
,
USP1
, and
HR3
, in
N. lugens
. These results indicate that the role of
CaMKII
in hemimetabolous insects may be different from that in holometabolous insects.
...
PMID:Molecular Characterization of Ca
2+
/Calmodulin-Dependent Protein Kinase II Isoforms in Three Rice Planthoppers-
Nilaparvata lugens
,
Laodelphax striatellus
, and
Sogatella furcifera
. 3122 88
