Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.13 (
protein kinase C
)
49,245
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A serine protease
prostasin
has been demonstrated to have a pivotal role in the activation of the epithelial sodium channel. Systemic administration of adenovirus carrying human
prostasin
gene in rats resulted in an increase in plasma
prostasin
and aldosterone levels. However, the mechanism by which the elevation of
prostasin
levels in the systemic circulation stimulated the plasma aldosterone levels remains unknown. Therefore, we examined if
prostasin
increases the aldosterone synthesis in a human adrenocortical cell line (H295R cells). Luciferase assay using CYP11B2 promoter revealed that
prostasin
significantly increased the transcriptional activity of CYP11B2. Prostasin significantly increased both CYP11B2 mRNA expression and aldosterone production in a dose-dependent manner. Surprisingly, treatment with camostat mesilate, a potent
prostasin
inhibitor, had no effect on the aldosterone synthesis by
prostasin
and also a protease-dead mutant of
prostasin
significantly stimulated the aldosterone production. A T-type/L-type calcium channel blocker and a
protein kinase C
(
PKC
) inhibitor significantly reduced the aldosterone synthesis by
prostasin
. Our findings suggest a stimulatory effect of
prostasin
on the aldosterone synthesis by adrenal gland through the nonproteolytic action and indicate a new role of
prostasin
in the systemic circulation.
...
PMID:Regulation of adrenal aldosterone production by serine protease prostasin. 2020 33
