Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.11.13 (protein kinase C)
 49,245 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We investigated the effect of topically applied diacylglycerols (DG) on melanogenesis in Skh-2 pigmented hairless mouse skin. Groups of mice were treated according to 4 different regimens of either 1,2-dioctanoyl-sn-glycerol (DOG) or 1-oleyl-2-acetyl-sn-glycerol (OAG) with or without ultraviolet irradiation (UVR). After the treatment regimens were completed, separated epidermal tissue was stained with L-dopa and thin sections of whole skin were stained by the Warthin-Starry method to detect melanin deposition. Quantification of the stained areas by digital image analysis disclosed that DOG treatment without UVR increased the dopa-positive area in skin in a dose-dependent manner but had no effect on melanin deposition. DG treatment acted synergistically with UVR to enhance melanogenesis, with synergism being more pronounced for melanin deposition than for dopa staining. DOG treatment prior to UVR also resulted in an enhanced melanogenic response to UVR, suggesting that DG increases the sensitivity of melanocytes to subsequent UVR by inducing dopa oxidase activity. OAG also enhanced UVR-induced melanogenesis in a dose-dependent manner and was at least as potent an inducer as was DOG. Because DG is known to activate protein kinase C, our results suggest that a protein kinase C-dependent process is involved in melanogenesis.
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PMID:Diacylglycerol-induced melanogenesis in Skh-2 pigmented hairless mice. 175 12

We studied the effect of N-(6-aminohexyl)-5-chloro-1-napthalenesulfonamide (W-7) on ultraviolet radiation (UVR)-induced melanogenesis (tanning) in Skh:HR2 pigmented hairless mice. Topically pretreated mice were exposed to subminimal edematogenic as well as edematogenic UVR doses to establish whether W-7-UVR-induced edema prophylaxis allows increased melanogenesis while preventing edema. Ultraviolet light-irradiated vehicle control animals developed visible tans; however, both W-7-treated groups failed to tan. Topical W-7 before UVR exposure inhibited UVR induction of dopa oxidase activity in melanocytes by 49% (P = 0.029) and inhibited UVR-induced deposition of melanin in the epidermis by 88% (P = 0.006). Topical W-7 blocked 23% of the UVR but this blockage could not account for the inhibition of dopa oxidase and melanization. We conclude that, in addition to preventing edema, W-7 inhibits UVR-induced melanogenesis, possibly by affecting Ca(2+)-calmodulin and/or protein kinase C-dependent processes.
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PMID:Topical W-7 inhibits ultraviolet radiation-induced melanogenesis in Skh:HR2 pigmented hairless mice. 885 Feb 46