Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.13 (
protein kinase C
)
49,245
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Hepatopulmonary syndrome
(
HPS
) is a serious complication in patients with advanced liver disease. The pathological pulmonary angiogenesis contributes to the progression of
HPS
. Importantly, directional collective migration of endothelial cells is a critical event for pathological angiogenesis. Previously, we have demonstrated that the over-expression of Cyclooxygenase-2 (COX-2) was an important factor in the experimental
HPS
. However, the role of COX-2 in the directional collective migration of human pulmonary microvascular endothelial cells (HPMVECs) is unclear. Our study aims to evaluate the potential effect of COX-2 in the directional collective migration of HPMVECs under the stimulation of
HPS
patient serum. In this study, 9 patients with stable liver cirrhosis were screened for presence of
HPS
. We confirmed that
HPS
patient serum dramatically promoted the directional collective migration and angiogenesis of HPMVECs, while the COX-2 selective antagonist parecoxib significantly inhibited the directional collective migration of HPMVEC under the stimulation of
HPS
patient serum. In addition,
HPS
patient serum significantly upregulated the phosphorylation of
PKC
and promoted the activation of Rac via COX-2/PGE2 signaling pathway. Notably, silencing
PKC
activation attenuated the directional collective migration of HPMVEC induced by
HPS
patient serum. In conclusion, these results indicate that
PKC
/Rac signaling induced by COX-2 modulates collective directional migration of HPMVEC during pathological pulmonary angiogenesis in
HPS
patients.
...
PMID:Cyclooxygenase-2 regulates HPS patient serum induced-directional collective HPMVEC migration via PKC/Rac signaling pathway. 3066 Jul 13
