Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.12 (
PKG
)
2,515
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In the mammalian brain, nitric oxide (NO) has been implicated in neuronal signal transmissions. NO stimulates guanylate cyclase to increase intracellular cGMP, which in turn activates cGMP-dependent protein kinases (
PKG
), but the targets of
PKG
in the brain have not fully been understood. In this study, we examined cGMP-dependent phosphorylation of proteins in rat brain and found that one of the possible substrates was myristoylated alanine-rich C-kinase substrate (MARCKS), an actin-binding membrane-associated protein that regulates cell adhesion. In addition, possible degradation products of MARCKS were observed after transfection of
PKG
or stimulation with 8pCPT-cGMP. Western blot analysis showed that the
MARCKS protein
levels were decreased when the cells were stimulated with 8pCPT-cGMP. These results suggest that MARCKS is a target of
PKG
, and
PKG
-dependent phosphorylation of MARCKS results in its degradation to reduce its protein levels in the cells.
...
PMID:cGMP-dependent phosphorylation and degradation of myristoylated alanine-rich C-kinase substrate. 1560 31
