Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.12 (
PKG
)
2,515
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Treatment of aortic smooth muscle cells with PDGF induces the upregulation of
protein tyrosine phosphatase 1B
(
PTP1B
).
PTP1B
, in turn, decreases the function of several growth factor receptors, thus completing a negative feedback loop. Studies have reported that PDGF induces the downregulation of
PKG
as part of a repertoire of dedifferentiation of vascular smooth muscle cells. Other studies have reported that chronic nitric oxide (NO) treatment also induces the downregulation of
PKG
. In the present study, we tested the hypothesis that the downregulation of
PKG
by PDGF or NO in differentiated rat aortic smooth muscle cells can be attributed to the upregulation of
PTP1B
. We found that treatment with PDGF or NO induced an upregulation of
PTP1B
levels. Overexpression of
PTP1B
induced a marked downregulation of
PKG
mRNA and protein levels, whereas the expression of dominant negative
PTP1B
or short interfering RNA directed against
PTP1B
blocked the capacity of PDGF or NO to decrease
PKG
levels. We conclude that the upregulation of
PTP1B
by PDGF or NO is both necessary and sufficient to induce the downregulation of
PKG
via an effect on
PKG
mRNA levels.
...
PMID:Suppression of PKG by PDGF or nitric oxide in differentiated aortic smooth muscle cells: obligatory role of protein tyrosine phosphatase 1B. 2105 40
