Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.11.11 (AMPK)
 12,425 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Neuronal control of the energy homeostasis requires the arcuate nucleus of hypothalamus. This structure integrates peripheral and central signals concerning the energy state of the body. It is comprised of two populations of neurons releasing anorexigenic and orexigenic peptides amongst others. Both populations are regulated by leptin, an anorexigenic hormone released by white adipose tissue. Voltage-gated calcium entry is critical to promote neurotransmitter and hormone release. It is already known that calcium channel current is inhibited by leptin in orexigenic neurons. However, fine-tuning details of calcium channel regulation in arcuate nucleus by leptin remain to be elucidated. This work aimed to investigate whether AMPK underlies the leptin-induced inhibition of calcium channels. By using patch-clamping methods, immunocytochemical and biochemical reagents we recorded calcium channel currents in orexigenic NPY neurons of arcuate nucleus of the rat. Consistently, leptin inhibition of the calcium channel current not only was prevented by AMPK inhibition with Compound C but also was hampered with AICAR. Furthermore, leptin inhibited selectively L-type calcium channel current amplitude without major changes in voltage dependence or current kinetics. These results support for the first time a key role of AMPK in the maintenance and regulation of the voltage-gated calcium channels. Together, they advance our understanding of the regulation of calcium channels in central nervous system and emerging questions concerning food intake and energy balance.
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PMID:AMPK mediates regulation of voltage-gated calcium channels by leptin in orexigenic neurons from arcuate nucleus. 3310 52


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