Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.11.11 (
AMPK
)
12,425
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Protein phosphatase Y (PPY) is a Drosophila testis-specific enzyme of unknown function. In a yeast two-hybrid screen we identified CG15031/
PPYR1
as a PPY interacting protein. The specificity of the protein-protein interaction was proven by directed two-hybrid tests. The complex formation between PPY and
PPYR1
was confirmed under in vitro and in vivo conditions by plasmon resonance spectroscopy, co-immunoprecipitation, and pull down experiments. Recombinant
PPYR1
expressed in Escherichia coli is a heatstable, protease sensitive, intrinsically unstructured RNA-binding protein that migrates anomalously in SDS-polyacrylamide gel electrophoresis. It can be phosphorylated by
cAMP-dependent protein kinase
in vitro.
PPYR1
moderately inhibits PPY activity, the inhibitory potential of the protein is slightly increased by phosphorylation. We suggest that
PPYR1
may function as a scaffolding protein that targets PPY to RNA and other protein partners in Drosophila melanogaster.
...
PMID:CG15031/PPYR1 is an intrinsically unstructured protein that interacts with protein phosphatase Y. 1663 Nov 4
