Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.11.1 (protein kinase)
 81,284 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Sporothrix schenckii induced sporotrichosis has gained importance in recent years because of its worldwide prevalence. The dimorphic switching process is required for the pathogenesis of S. schenckii. Previously, we found that STE20-like protein kinase (SsSte20) was overexpressed in the early yeast stage, but not in the mycelial stage of S. schenckii, which suggested its involvement in morphogenesis of this fungal pathogen. It remains unclear, however, whether SsSte20 is essential for dimorphic switching of S. schenckii and what are its related genes. In this study, the function of SsSte20 was investigated using double-stranded RNA interference (dsRNAi) mediated by Agrobacterium tumefaciens. We evaluated its effects on normal asexual development, yeast-phase cell formation, and cell wall composition and integrity. In addition, by transcriptome analysis of the SsSte20 knockdown (SsSte20-i) mutant and the standard S. schenckii strain, we further investigated the genes and pathways that were affected by SsSte20. Our results showed that inactivation of SsSte20 significantly affected the growth and internal components of S. schenckii conidia and impaired the dimorphic switching process. RNA transcriptome analysis of the standard S. schenckii strain and the SsSte20-i mutant revealed that SsSte20 inhibition affected the genes that were not only involved in the biological process, but also in the cellular component, and the molecular functions of S. schenckii. It mainly affected the expression of iron/ion-binding transporter genes, oxidation-reduction-related genes, 1, 3-beta-glucosidase, and methylsterol monooxygenase, which are highly associated with environmental information processing and the biosynthesis of cell wall components. Overall, our research supports the claim that SsSte20 plays an essential role in the dimorphism of S. schenckii and affects its global transcriptome.
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PMID:Ste20 is crucial for dimorphic switching of sporothrix schenckii and affects its global transcriptome. 3226 78