Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.10.2 (focal adhesion kinase)
 44,029 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Cryostat sections of normal skin from 57 white adults were examined by direct and indirect immunofluorescence for immunoglobulins, complement factors, and transferrin. The results for basement membrane zone (BMX) were significantly different for the 11 face and 46 non-face biopsies: in the face, IgM was found in five, IgG in two, IgA in one, and C3 in none, whereas, in non-face, IgM was present in six, IgG in none, IgA in one, and C3 in five. The results for dermal vessel walls (DV) were not apparently different for face and non-face; in the 57 biopsies IgM was present in one, IgG in none, IgA in one, and C3 in one. The 11 biopsies from the face and 26 of the non-face biopsies were examined further. No IgD or C4 was identified, but one case (scalp) showed BMZ Clq, properdin, and transferrin, and in two cases (one face, one non-face) DV properdin was found. Cytoid bodies (IgM and IgA) were present in moderate numbers in one case; all other positive reactions were finely granular.
J Clin Pathol 1978 Dec
PMID:Immunoglobulin and complement in normal skin. 37 44

Before and after heat acclimation, four male resting subjects were exposed to humid heat that caused levels of skin wettedness ranging from 50 to 100%. The physical experimental conditions were chosen so that the same skin wettedness was attained with modification of only the ambient water vapor pressure, at two wind speeds (0.6 and 0.9 m . s-1). The esophageal temperature (Tes), mean skin temperature (Tsk), sweating rate (msw), and dripping sweat rate (mdr) were recorded; the amounts of local drippage in the same thermal conditions before and after acclimation were also determined. The relationship between the evaporative efficiency of sweating (eta sw) and the skin wettedness (w) is reported, as is the influence of the subject's acclimation to humid heat on adjustments of skin wettedness. The effects of the air velocity on the coefficient of evaporation and on sweating efficiency are discussed. Beneficial increases in evaporation were achievable by increasing skin wettedness only when there was a consistent drippage, which differed from one body area to another and from one subject to another. The relation of drift in body temperature to skin wettedness changed with the acclimation of the subjects.
J Appl Physiol Respir Environ Exerc Physiol 1979 Dec
PMID:Influence of air velocity and heat acclimation on human skin wettedness and sweating efficiency. 53 89

A method has been developed for freezing, drying and embedding of unfixed monolayer cultures for electron microscopic autoradiography (EM ARG). Experimental results showed: a) Aclar 33 C was a more suitable substrate than the plastic of petri dishes, b) cultures pressed rapidly against the polished face of a large copper cylinder chilled in liquid nitrogen had better cellular morphology than did cultures dipped in Freon 12 chilled in liquid nitrogen, and c) cultures embedded in Epon alone had finer extracellular ice spaces and lower background grain densities than did cultures embedded in Epon with 1% silicone. This method has been used to evaluate the effect of fixation on the localization of the neurotransmitter, 3H-gamma-aminobutyric acid (3H-GABA), in neurons of dispersed cell cultures. EM ARG results showed that the neuronal cell bodies and vesicle elements were present in similar numbers in both glutaraldehyde fixed and freeze-dried cultures.
Histochemistry 1978 Dec 13
PMID:Freeze-drying of unfixed monolayer cultures for electron microscopic autoradiography. 57 Jan 82

At 43 degrees C (but not at 41 degrees C), organic solvents used to dissolve water-insoluble chemotherapeutic agents become themselves lethal to cells. This finding is not unique to Chinese hamster cells (HA-1); mouse mammary sarcoma cells (EMT-6) behave similarly. The solvent concentrations involved are in the range of those needed to make drug solutions. Hence experiments measuring drug-cell interactions at elevated temperatures must include controls which independently measure solvent effects.
J Cell Physiol 1977 Dec
PMID:Cytotoxicity of commonly used solvents at elevated temperatures. 59 66

The light-activated, nucleic acid-binding drugs, psoralens, were used in conjunction with a 365-nm laser microbeam to selectively bind to any nucleic acids in the centriolar region. 4'-aminomethyl-4,5',8--trimethyl-psoralen (AMT) has a high affinity for both RNA and DNA and can be shown to cause mitotic abortion when centriolar regions of prophase PTK2 cells and reacted with AMT and 365-nm laser light. Other psoralen derivatives which have a high affinity for DNA and a low affinity for RNA are not effective in blocking mitosis in dividing PTK2 cells. Examination of psoralen-bound centriolar regions by single-cell electron microscopy shows that at various times after treatment, the number of microtubules associated with the irradiated poles is much lower than in normal, dividing cells. Light-activated psoralen binding of the centriolar regions does not seem to affect the condensation or structure of mitotic chromosomes. It is concluded that there is an RNA in the centriolar region that is responsible for the formation of the spindle in dividing cells.
J Cell Sci 1978 Dec
PMID:Evidence for centriolar region RNA functioning in spindle formation in dividing PTK2 cells. 74 44

Mice of 1.5, 9, 22, and 31 to 32 months of age were injected with the thymus-dependent antigen, TNP-SRC, or the thymus-independent antigen, TNP-SRC, TNP-MRC. The anti-SRC and TNP immune responses to TNP-SRC were markedly reduced in older mice, whereas the anti-TNP response to the TNP-MRC showed no substantial decline. Young mice produced higher anti-TNP plaque-forming cell responses after injection of TNP-SRC than after TNP-MRC, whereas in older mice the reverse obtained. Old mice but not young mice displayed a high anti-SRC cross-reactive response after injection of TNP-MRC. The avidity of anti-TNP antibody of young mice immunized with TNP-SRC was higher than that following immunization with TNP-MRC, whereas the avidities of anti-TNP antibodies from old mice injected with these two reagents were the same. Those individual mice which showed a poorly regulated immune response also displayed an autologous anti-MRC plaque-forming cell response after injection of either TNP-SRC or TNP-MRC. It is suggested that mechanisms mediated by suppressor T cells may be responsible for regulating the autoimmune response to modified self antigens, and that these are severely impaired in age individuals.
J Immunol 1976 Dec
PMID:Autoimmunity and aging: the age-related response of mice of a long-lived strain to trinitrophenylated syngeneic mouse red blood cells. 79 42

The centriole regions of prophase PTK2 cells were irradiated with a laser microbeam. Cells continued through mitosis normally. Ultrastructural analysis revealed either an absence of centrioles or severely damaged centrioles at the irradiated poles. Microtubules appeared to focus into pericentriolar cloud material.
J Cell Biol 1977 Dec
PMID:Continuation of mitosis after selective laser microbeam destruction of the centriolar region. 92 90

To judge standard practice for managing arrhythmias, what an EMT should be able to do in the field, drugs of choice, the success of EMT training, and the quality of EMT performance, clinical algorithms were developed. Branching logic, forcing yes/no decisions and delineating actions for all contingencies helped formalize and systematize EMT management of urgent and emergency cases. The algorithm set was sent to 19 consultants for review of content, sequence, drug dosage, and drug usage. The results indicated lack of consensus on appropriate prehospital cardiac care, but the approval of the algorithm approach.
JACEP 1977 Dec
PMID:Prehospital cardiac care: illusion of consensus. 92 13

Nephron functions of an improved isolated perfused rat kidney preparation were studied by micropuncture techniques. Single-nephron glomerular filtration rate (SNGFR), intratubular pydrostatic pressures (IP), transit time (TT), and the reabsorption (R) of H2O, Na, Cl, and K were measured in superficial proximal (PT) and distal tubules (DT) of the preparation. Mean SNGFR was 27.2 nl/min and 25.2 nl/min when measured in PT and DT, respectively. The PT transport functions were well maintained throughout the perfusion (mean values were: IP, 14.3 mmHg; TT, 17.7 s; fractional (F) RH2O, 64%; absolute RH2O, 15.4 nl/min; FRNA, 66.5%; FRK, 71%, and tubular fluid-to-perfusate tf/p) ratio of Cl, 1.37). The short loops of Henle reabsorbed less than 10% of the load of H2O and Na delivered to them and the TF/P ratio of electrolytes in the earliest DT segments were high (TF/P)Na = 0.88, (TF/P)Cl = 1.27, and (TF/P)K = 1.11). This deficiency in function of Henle's loop explains, at least in part, the degree of natriuresis of the preparation (overall FRNa = 97.5%). Transit time to end DT was prolonged (82.3 S) and IP in DT elevated (14.9 mmHg). The DT was able to compensate, in part, for the overload from Henle's loop by reabsorbing 36% of the fluid load and 54% of the Na load delivery to it. We concluded that the improved isolated perfused rat kidney is a suitable preparation with which to study several aspects of renal function, particularly proximal tubules transport functions.
Am J Physiol 1976 Dec
PMID:Nephron function of the isolated perfused rat kidney. 99 Jan 8

A three-part experiment was designed to examine interactions between local and reflex influences on forearm skin blood flow (SkBF). In part I locally increasing arm skin temperature (Tsk) to 42.5 degrees C was not associated with increases in underlying forearm muscle blood flow, esophageal temperature (Tes), or forearm blood flow in the contralateral cool arm. In part II whole-body Tsk was held at 38 or 40 degrees C and the surface temperature of one arm held at 38 or 42 degrees C for prolonged periods. SkBF in the heated arm rose rapidly with the elevation in body Tsk and arm Tsk continued to rise as Tes rose. SkBF in the arm kept at 32 degrees C paralleled rising Tes. In six studies, SkBF in the cool arm ultimately converged with SkBF in the heated arm. In eight other studies, heated arm SkBF maintained an offset above cool arm SkBF throughout the period of whole-body heating. In part III, local arm Tsk of 42.5 degrees C did not abolish skin vasoconstrictor response to lower body negative pressure. We conclude that local and reflex influences to skin interact so as to modify the degree but not the pattern of skin vasomotor response.
J Appl Physiol 1976 Dec
PMID:Interactions between local and reflex influences on human forearm skin blood flow. 100 38


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