Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.10.2 (
focal adhesion kinase
)
44,029
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Purpose
: The function of
Solcoseryl
in the corneal epithelium has not been fully examined. Here, we investigated the roles of
Solcoseryl
in the regulation of gene expression and corneal epithelial cell (CEC) activity.
Materials and Methods
: The effect of
Solcoseryl
on CEC activity was analyzed through cell migration, adhesion, proliferation, and wound healing assays. Analysis of gene expression was conducted via western blotting and quantitative reverse transcription polymerase chain reaction (PCR).
Results
: The results demonstrated that
Solcoseryl
increased the adhesion, migration, proliferation, and wound healing of CECs. Analysis of gene expression showed that
Solcoseryl
-stimulated CECs exhibited increased expression of mucin family genes, such as
MUC1
, -5
AC
, -7, and -1
6
.
Solcoseryl
also increased the activities of the intracellular signaling molecules AKT,
FAK
, ERK, and Src in CECs. Using pharmacologic inhibitors of ERK and AKT, we showed that the expression of mucin genes by
Solcoseryl
is mediated by the activation of ERK and AKT signaling.
Conclusions
: Our findings demonstrate that
Solcoseryl
may contribute to the wound healing of CECs by enhancing their migration, adhesion, and proliferation. Additionally, our results suggest that
Solcoseryl
has a protective effect on ocular surfaces due to its induction of the expression of mucin genes in CECs. These findings suggest that
Solcoseryl
is a useful therapeutic target for patients with corneal wounds.
...
PMID:Wound Healing and Mucin Gene Expression of Human Corneal Epithelial Cells Treated with Deproteinized Extract of Calf Blood. 3120 24
