Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:2.7.10.2 (focal adhesion kinase)
44,029 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In this work we present a graphite furnace atomic absorption spectrometric method for blood lead using palladium as a chemical modifier. Whole blood was diluted 10- fold with a 0.1% v/v triton X-100 solution; 10 microL of this solution and 10 microL of the palladium-based modifier (2 mg Pd/L, 2% w/v citric acid and 0.01 M nitric acid) were injected onto the L'vov platform by using the alternate volume mode. The following furnace operating parameters were used: (a) drying steps, 120 degrees C for 10s and 250 degrees C for 30s; (b) pyrolysis steps, 800 degrees C for 45s (with oxygen) and 1100 degrees C for 25s; (c) atomization, 1600 degrees C for 3s; (d) clean out, 2700 degrees C for 4s. Accuracy was tested by using (i) a NIST standard (SRM-909) and the Behring Control Blood for Metal 1 (OSSD 21) with lead concentrations of 23.7 +/- 2.1 micrograms/L (found: 21.2 +/- 0.7 micrograms/L) and 413 +/- 51 micrograms/L (found: 407 +/- 6 micrograms/L), respectively; (ii) recovery studies (ca. 100 +/- 1%), and (iii) a reported method (mean relative error: 5.1%). Approximate standard deviations of 0.3 (within-run) and 0.7 (between-runs) micrograms Pb/L were found in the precision study. The detection limit (3 sigma) and the characteristic mass (for a 10- microL injection volume) were 0.1 micrograms Pb/L and 15 pg/0.0044 A.s, respectively. The proposed method was used to establish the lead levels of patients with renal insufficiency; a mean concentration (+/- SD) of 59 +/- 39 micrograms Pb/L (range: 12- 160 micrograms Pb/L) was found. The method was interference-free, reliable and reproducible.
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PMID:Graphite furnace atomic absorption spectrometric determination of blood lead with palladium modification. 193 67

Muscle tone was tested at the shoulders and wrists of 49 randomly selected poststroke patients with the use of resting joint position (SJP and WJP), resistance to passive movement or stiffness (SRM and WRM), and angle of appearance of resistance (SAR and WAR). Subjects were tested while seated with their arm supported in a suspension sling adapted for free movement. Five of the first and immediately repeated measurement pairs showed strong correlations and interrater reliability (SJP, .839; WJP, .900; SRM, .886; WRM, .904; SAR, .884 [p less than .05]). The sixth (WAR) showed moderate reliability (.618, p less than .05). Resting joint position measurements were most reliable among subjects with higher tone. The joint first measured had a slight order effect on SRM among subjects with higher muscle tone. Its second measurements were slightly increased over the first among those subjects whose shoulders were measured first and slightly reduced when measured immediately after the wrist. Reliable means of clinical evaluation of muscle tone at the shoulder and wrist are available if the influence of level of tone and the mutual influence of muscles tested are prudently considered.
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PMID:Reliability of three clinical measures of muscle tone in the shoulders and wrists of poststroke patients. 200 Sep 24

The Sociomoral Reflection Measure (SRM; Gibbs & Widaman, 1982) was developed as a group administerable instrument for measuring developmental stages of moral reasoning. The aim of this study was to examine its reliability and construct validity, employing a sample of 542 Swedish elementary and high school students (aged 8-17), from schools within metropolitan Stockholm. Interrater reliability varied between 0.83 and 0.92, and the internal consistency coefficient was 0.76. Factor analyses of the norms (on which the overall score is based) revealed one factor for the oldest age group (16-17 years), but two factors among the younger subjects (8-15 years), clearly separating the norms related to each of the two moral dilemmas. The Sociomoral Reflection Maturity Score was moderately, but significantly, related to both age and grade. The results suggests that, although used in a non-American context and with self-trained raters, the SRM seems highly applicable in Sweden, at least for research purposes.
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PMID:The sociomoral reflection measure: applicability to Swedish children and adolescents. 204 96

The conditions were evolved and checked for simultaneous determination of cadmium and lead levels in plant material using the flame technique of ASA. For decomposition of the organic substances in plant material wet mineralization was used with a mixture of nitric acid, perchloric acid and sulpuric acid in volume proportions 6:2:0.25. The levels of cadmium and lead were determined in the organic phase after extraction with n-butyl acetate of the previously produced complexes with NaDDTK. The obtained limits of cadmium and lead detectability were 0.002 and 0.02 mg/kg respectively. The recovery rate of the method ranged from 96 to 98%, while the variability index was from 2.6 to 10.2%. The correctness of the evolved analytical procedure was confirmed by determination of the content of both elements in the NBS-SRM 1571 standard (orchard leaves) and by participation in the international interlaboratory investigation of the Polish standard (dried cabbage leaves).
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PMID:[A method of atomic absorption spectrophotometry (AAS) for analysis of cadmium and lead levels in the plant material]. 210 Nov 73

The urine level of trace elements has been investigated in some normal adults under different workplace environments in Bangladesh. The samples were collected from 58 subjects, randomly selected from about 500 adults working in 10 different workshops and factories located in Dhaka city. Samples were analyzed by the external beam PIXE method. Proton beams of 2.5 MeV energy and 20 nA intensity were used for sample irradiations. For 40 microC irradiations, the concentration of 14 elements in total (K, Ca, Ti, Cr, Mn, Fe, Ni, Cu, Zn, As, Br, Rb, Sr and Mo) in different groups were measured by comparison with a calibration curve obtained from the NBS Orchard Leaf standard (SRM 1571). The results from different workplaces are reported in terms of amount of excretion per unit volume of the sample collected. They, however, do not reflect the total daily excretion level of different subject groups.
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PMID:Urine level of trace elements in some adults in Bangladesh under a workplace environment. 215 44

An isotope dilution gas chromatography/mass spectrometry method using lithium bis(trifluoroethyl)dithiocarbamate as a chelating agent is described for the determination of chromium in urine. A wet digestion procedure with HNO3-H2O2 is used for oxidizing the organic matter associated with urine samples. The isotope ratios are measured by selected ion monitoring in a general-purpose mass spectrometer using a 10-m fused silica capillary column. Memory effect, in sequential analyses of samples with different isotope ratios, was evaluated by preparing a series of synthetic mixtures and was found to be negligible. The accuracy of the method was verified by quantitation of chromium in the NIST freeze-dried urine reference material, SRM-2670, with a recommended chromium concentration of 13 micrograms/L in the normal level and certified chromium concentration of 85 +/- 6 micrograms/L in the elevated level.
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PMID:Determination of chromium in urine by stable isotope dilution gas chromatography/mass spectrometry using lithium bis(trifluoroethyl)dithiocarbamate as a chelating agent. 217 91

The technique of laser resonance ionization mass spectrometry has been combined with isotope dilution analysis to determine iodine in oyster tissue. The long-lived radioisotope, 129I, was used to spike the samples. Samples were equilibrated with the 129I, wet ashed under controlled conditions, and iodine separated by coprecipitation with silver chloride. The analyte was dried as silver ammonium iodide upon a tantalum filament from which iodine was thermally desorbed in the resonance ionization mass spectrometry instrument. A single-color, two-photon resonant plus one-photon ionization scheme was used to form positive iodine ions. Long-lived iodine signals were achieved from 100 ng of iodine. The precision of 127I/129I measurement has been evaluated by replicate determinations of the spike, the spike calibration samples, and the oyster tissue samples and was 1.0%. Measurement precision among samples was 1.9% for the spike calibration and 1.4% for the oyster tissue. The concentration of iodine determined in SRM 1566a, Oyster Tissue, was 4.44 micrograms/g with an estimate of the overall uncertainty for the analysis of +/- 0.12 microgram/g.
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PMID:Determination of iodine in oyster tissue by isotope dilution laser resonance ionization mass spectrometry. 217 92

Analytical performance of the Boehringer Mannheim/Hitachi 717 system was evaluated in a multicenter study involving seven different laboratories. Fifty-five methods including end point chemistries, enzymes, ISE, TDM, DAU, and specific protein assays were assessed over a 7 month period. Methods on the analyzer exhibited excellent precision with CVs less than 2% for within run precision, and CVs less than 3% for between day precision for most analytes; linearity, which met or exceeded manufacturer's claims; minimal sample and reagent carryover, and no significant interference from hemolysis; icterus; and lipemia. Recovery of the assigned value for 10 analytes in SRM 909 was acceptable. Comparison of methods with other BM/Hitachi analyzers resulted in slopes close to unity (0.93-1.06); comparison to other clinical chemistry analyzers yielded slopes of 0.88-1.07. Excellent performance and diverse method applications make the BM/Hitachi 717 analyzer a suitable instrument for work station consolidation.
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PMID:A multicenter evaluation of the Boehringer Mannheim/Hitachi 717 system. 228 5

We report a case of periodic leg movements (PLM) observed in an 86-year-old man during either midthoracic epidural anesthesia or spinal anesthesia. The PLM observed were stereotyped (extension of the big toe in combination with partial flexion of the ankle, knee, and hip lasting 3-5 s) and repetitive (interevent intervals between jerks were 20-40 s) for about 120 and 30 min respectively. The patient was awake but unaware of the PLM unless reminded. The present case was quite similar to sleep-related (noctural) myoclonus (SRM) in every respect except for its occurrence during wakefulness. SRM is more prevalent in the elderly population but its mechanism remains to be elucidated. Previously, we had reported a case of PLM observed in an elderly man with SRM. In our two cases, PLM were seen only while the local anesthetic was acting on the spinal cord; therefore, these anesthesia-related PLM (ARPLM) may suggest that the spinal cord is involved. In particular, we consider that physiological changes seen commonly during non-rapid-eye-movement sleep and a certain phase of anesthesia, such as suppression of the descending inhibitory pathway, and pyramidal tract dysfunction are relevant to ARPLM. In addition, the concomitant alteration of the blood flow in the leg and changes due to aging of the spinal cord may also be involved.
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PMID:Periodic leg movements during either epidural or spinal anesthesia in an elderly man without sleep-related (nocturnal) myoclonus. 235 97

In recent years, laboratory testing in the critical-care setting has increased, a trend due, in part, to the evolution of electrochemical sensors. Various innovations have extended sensor lifetimes, reduced sensor maintenance, and led to the development of single-use and unit-use disposable sensors. These sensor technologies allow the accurate and precise determination, either at or near the bedside, of several analytes including pO2, pCO2, pH, Na, K, Cl, ionized calcium, hematocrit, total hemoglobin, and glucose. Use of these new systems, however, has raised new issues regarding sensor calibration and sample handling and collection. The number of direct-reading analyzers for electrolyte determinations has also increased dramatically. Issues regarding calibration of ion-selective electrodes (ISEs) for Na/K have also been raised after demonstrations of between-instrument variation. Recently, collaborative efforts between eight ISE instrument manufacturers and the National Institute of Standards and Technology resulted in the development of a Standard Reference Material, SRM 956, for the purpose of standardizing direct-reading Na/K ISEs to the flame photometer. Other widely used technologies that provide noninvasive, continuous monitoring include pulse oximetry and transcutaneous gas electrodes. These trends are expected to continue and to produce a new generation of electrochemical and optical sensors.
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PMID:Current analytical approaches to measuring blood analytes. 238 68


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