Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.10.1 (ERK)
 95,504 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The incidence of genetic abnormalities have been investigated in a variety of preleukaemic states RAS and FMS oncogene, p53 suppressor gene mutations and monoclonality in myelodysplastic syndromes (MDS), a paradigm for pre-leukemias have been observed. Other patients at risk of developing either secondary leukaemia or evolving into leukaemia have been similarly studied including haematologically normal patients in remission from lymphoma. Time from treatment to detection of genetic abnormalities is a significant factor in some of these patients which is consistent with the expansion of an abnormal clone. A case of non-dysplastic MDS has been identified with a 7q-karyotypic abnormality typical of therapy related MDS, abnormal progenitor growth and RAS mutations but with normal clinical features. Normal individuals have also been under investigation and found to have a low incidence of proto-oncogene mutations. A prospective study should enable us to determine if these parameters are indeed prognostic indicators.
Acta Haematol Pol 1993
PMID:Genetic lesions in preleukemia. 824 36

During 1981-90, there were 141 births to women under the age of 18 from among 22,037 deliveries at the II Department of the Obstetrical and Gynecological Clinic of the Academy of Medicine in Warsaw. The control group consisted of 141 consecutive primiparas aged 19-30. Among the 22,037 births, the underage women accounted for 0.64%. 10 women were under 16 years of age, and two patients each were 13 and 14 years of age, respectively, while 23 were aged 16, and 108 were aged 17. The percentage of pregnant adolescents increased from 0.13% in 1982 to 0.84% in 1986 and to 1.24% in 1990. There were 47 (33.67%) pregnancy complications among adolescents and 55 (39%) among adults. The threat of premature birth occurred in 11 adolescents (7.0%) vs. 13 adults (9.22%); premature discharge of amniotic fluid in 21 adolescents (14.89%) and 25 adults (17.73%); EPH gestosis in 4 adolescents (2.84%) and 5 adults (3.55%); infection of the urinary tract in 7 adolescents (4.96%) and 10 adults (7.10%). Normal delivery occurred in 124 (87.94%) adolescents and in 112 (79.4%) adults. Cesarean sections were performed in 11 (7.8%) adolescents and 24 (17.1%) adult primiparas. There were 26 (18.4%) instances of delivery complications in adolescents and 66 (46.7%) in adults. Incomplete delivery occurred in 21 (14.9%) adolescents vs. 23 (16.3%) adults. Significantly more (39) cases of rupture of the uterine cervix occurred in adults (27.6%) than in adolescents (4 instances or 2.8%). The duration of labor ranged from 2 hours to 12.5 hours in the group of adolescents with an average of 7.1 hours compared to from 2 hours to 25 hours with an average of 7.9 hours in the control group. The average values in the first minute of life amounted to 8.85 marks on the Apgar score in the case group compared to 9.36 scores in controls. The average birth weight of neonates was 3190 g in the case group compared to 3284 g in the control group.
Ginekol Pol 1993 Aug
PMID:[Analysis of the course of pregnancies and labor in girls under 18 years of age]. 837 22

TIF-IB is a transcription factor which interacts with the mouse ribosomal gene promoter and nucleates the formation of an initiation complex containing RNA polymerase I (Pol I). We have purified this factor to near homogeneity and demonstrate that TIF-IB is a large complex (< 200 kDa) which contains several polypeptides. One of the subunits present in this protein complex is the TATA-binding protein (TBP) as revealed by copurification of TIF-IB activity and TBP over different chromatographic steps including immunoaffinity purification. In addition to TBP, three tightly associated proteins (TAFs-I) with apparent molecular weights of 95, 68, and 48 kDa are contained in this multimeric complex. This subunit composition is similar--but not identical--to the analogous human factor SL1. Depletion of TBP from TIF-IB-containing fractions by immunoprecipitation eliminates TIF-IB activity. Neither TBP alone nor fractions containing other TBP complexes are capable of substituting for TIF-IB activity. Therefore, TIF-IB is a unique complex with Pol I-specific TAFs distinct from other TBP-containing complexes. The identification of TBP as an integral part of the murine rDNA promoter-specific transcription initiation factor extends the previously noted similarity of transcriptional initiation by the three nuclear RNA polymerases and underscores the importance of TAFs in determining promoter specificity.
 ...
PMID:A TBP-containing multiprotein complex (TIF-IB) mediates transcription specificity of murine RNA polymerase I. 841 71

Previously we have shown that the RNA polymerase I (Pol I)-specific transcription factor UBF stimulates transcription by both facilitating transcription complex formation and by relieving repression exerted by a negative-acting factor which competes for binding of the murine factor TIF-IB to the ribosomal gene promoter (1). We have purified and functionally characterized this repressor protein from Ehrlich ascites cells. The final preparation contained two polypeptides with molecular masses of 75 and 90 kDa, respectively. Both polypeptides interact with the rDNA promoter as revealed by UV-crosslinking experiments. The specificity of binding to the ribosomal gene promoter was demonstrated in an electrophoretic mobility shift assay and by DNase footprinting. The biochemical properties of this negative-acting factor closely resemble those of the Ku antigen, a human nuclear DNA-binding heterodimer which is the target of autoantibodies in several autoimmune diseases. Anti-Ku antibodies precipitate the repressor activity and overcome transcription inhibition. The data demonstrate that regulation of Pol I gene transcription may involve an antirepression mechanism as already documented for Pol II genes and suggest that Ku protein may be causally involved in repressor-mediated down regulation of rRNA synthesis.
 ...
PMID:The nucleolar transcription activator UBF relieves Ku antigen-mediated repression of mouse ribosomal gene transcription. 850 46

An intrinsic property of class I gene transcription by RNA polymerase I (Pol I) is the species specificity of the initiation reaction. Previous studies have demonstrated that species-specific rDNA promoter recognition is brought about by a TBP-TAF complex, termed TIF-IB in mouse and SL1 in man. We have compared the ability of affinity-purified TIF-IB and SL1 to direct transcription from the homologous rDNA template both in a reconstituted transcription system and in nuclear extracts prepared from mouse and human cells. We show that Pol I from both species and the individual transcription factors, with the exception of TIF-IB/SL1, are functionally interchangeable in the reconstituted transcription system containing purified proteins. In nuclear extracts, however, species-specific differences are obvious. Whereas SL1 reprograms a heterologous mouse extract to recognize the human promoter, TIF-IB fails to reprogram a human extract unless it is complemented with mouse Pol I. Crude human, but not mouse, Pol I exhibits species-specific differences that disappear after purification. We propose that in extracts and less purified fractions human Pol I exists as 'holoenzyme' containing associated protein(s) that prevent assembly of TIF-IB-directed initiation complexes at the murine rDNA promoter.
 ...
PMID:Species specificity of ribosomal gene transcription: a factor associated with human RNA polymerase I prevents transcription of mouse rDNA. 863 44

The study was performed on 70 pregnant women, divided into two groups: normal or EPH gestosis. The concentration of serotonin in plasma was determined according to Manuchin et al., the activity of monoamine oxidase was determined using the radioisotope method of Yuodim. Daily urinary excretion of 5-hydroxyindoleacetic acid was established with the method of Udenfriend et al. It was found that women with EPH gestosis have significantly higher (p < 0.01) plasma concentration of serotonin and significantly lower activities of MAO (p < 0.001) in serum and in blood platelets and urinary excretion of 5-hydroxyindoleacetic acid (p < 0.05) than normal pregnant women.
Ginekol Pol 1995 Aug
PMID:[Levels of serotonin in plasma and activity of monoamine oxidase in serum and blood platelets of women with EPH gestosis]. 867 66

The association of the polymorphisms of two candidate genes with essential hypertension was studied in 74 hypertensive and 118 normotensive subjects. Two restrictions endonucleases were used: PstI for the insulin receptor gene and PvuII for the apolipoprotein B gene. PstI RFLP in the INSR gene locus consists of two polymorphic alleles P1 (1800bp) and P2 (1500bp). Frequencies of these alleles in general population are 0.15 and 0.85 respectively. The results showed statistically significant association between P1 allele and homozygotus genotype P1P1 for the INSR gene and essential hypertension. Clinical data of homozygotus P1P1 individuals revealed earlier clinical onset and more severe course of the disease. PvuII RFLP in the apoB gene locus consists of two polymorphic alleles Pul (7900bp) and Pu2(5500 bp). Frequencies of these alleles in general population are 0.93 and 0.07 respectively. In the apoB gene analysis Pu1 and Pu2 allele frequencies were similar in both studied groups. However the higher frequency of homozygotus genotype Pu1Pu2 was observed in hypertension.
Pol Arch Med Wewn 1996 Mar
PMID:[Polymorphic variability of apolipoprotein B genes and insulin receptor in essential hypertension]. 875 50

The influence of a new discovered haematopoietic growth factor known as ligand of STK-1 receptor (FLK2/FLT3) on growth of human erytropoietic progenitors in vitro was evaluated. Studies were performed on bone marrow cells enriched in haematopoietic progenitors expressing CD 34 antigen in serum supplemented as in serum free medium. In conclusion STK-1 receptor ligand (STK-1L) does not influence the growth of human erythroid progenitors in vitro. Therefore STK-1L would not find practical application in future in vivo therapy as erythropoiesis stimulatory agent.
Pol Arch Med Wewn 1995 Nov
PMID:[The effect of STK-1 receptor (FLK2/FLT3) ligand on human erythropoiesis in vitro. Clinical implications]. 883 39

Efficient transcription elongation by RNA polymerase I (Pol I) requires a specific Pol I-associated factor, termed TIF-IC. Here we show that TFIIS, a factor that has previously been shown to promote read-through past many types of blocks to elongation by RNA polymerase II, also enhances Pol I-directed transcription elongation. In a reconstituted transcription system containing purified proteins, TFIIS stimulates Pol I transcription by increasing the overall rate of RNA chain elongation. As with Pol II, ternary Pol I complexes cleave the 3' end of the nascent transcripts in response to TFIIS. The truncated RNAs remain bound to the template, are subject to pyrophosphorolysis, and can be chased into longer transcripts. Moreover, we show by immunoprecipitation and specific affinity chromatography that TFIIS physically interacts with Pol I. The results suggest that nascent transcript cleavage by TFIIS or a TFIIS-related factor may be a general mechanism by which both Pol I and Pol II can bypass transcriptional impediments.
 ...
PMID:TFIIS binds to mouse RNA polymerase I and stimulates transcript elongation and hydrolytic cleavage of nascent rRNA. 887 42

The analysis covered 61 women with a history of EPH gestosis and 63 children born to them as well as 30 healthy women with normal arterial blood pressure during pregnancy and their 30 children aged 3-8 years. Hypertension exceeding 145/95 mm Hg was found in 26 mothers out of 61 at the moment of examination. In the other 35 patients from the EPH gestosis group arterial blood pressure was still within the norm, but diastolic blood pressure (DBP) values were significantly higher than in the control group. The values of DBP in children from mothers with EPH gestosis were higher than the values of DBP in children from the control group. However significantly higher values were observed only in children from mothers with constantly elevated blood pressure.
Ginekol Pol 1996 May
PMID:[Arterial blood pressure 3-8 years after EPH gestosis in mothers and their children]. 892 93


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>