Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.10.1 (
ERK
)
95,504
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The prostate transglutaminase,
TGase-4
, is a member of the transglutaminase family and is uniquely expressed in the prostate gland. The function of the protein is largely unknown, although an influence on cell motility and adhesion has been indicated. The present study investigated the impact of the differential expression of
TGase-4
in human prostate cancer cells on
RON
, the hepatocyte growth factor-like/macrophage-stimulating protein (HGF-L/MSP) receptor, mediated cellular functions. Using human prostate cancer cell lines and prostate tissues, we demonstrated that human
TGase-4
had a high degree of co-localisation with
RON
, primarily at the cell periphery and cell-cell adhesion region. High levels of
TGase-4
expression in CAHPV10 cells and in PC3 cells engineered to over-express
TGase-4
were associated with significantly increased cell motility in response to HGF-L, a clear contrast to wild-type and control cells. Neutralising antibody to
RON
and rhHGFL/MSP had no further bearing on the increased motility in
TGase-4
over-expressing cells, although they had profound effect on the control cells. Akt pathway inhibitor significantly diminished the effect induced by HGF-L in the cells. Finally, over-expression of
TGase-4
in prostate cancer cells resulted in autophosphorylation of
RON
. It is concluded that
TGase-4
expression is intrinsically linked to the activation of
RON
in prostate cancer cells and that this autoactivation of
RON
contributes to the increased cell motility in
TGase-4
expressing cells.
...
PMID:The prostate transglutaminase, TGase-4, coordinates with the HGFL/MSP-RON system in stimulating the migration of prostate cancer cells. 2059 68
