Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.7.10.1 (ERK)
 95,504 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

HEP Flury strain of rabies virus was propagated in chick embryo cells under maintenance media of different pH. It was found that viral growth was better and reached a markedly higher maximum titer when the initial pH of maintenance medium was 8.2 to 9.0 than when it was 7.4. The enhancement of viral growth was not ascribable to mere neutralization of acids produced from infected cells, because the different media became almost equally neutral within an early phase of growth curve. Serial passage of the virus in chick embryo cells using pH 8.2 maintenance medium resulted in altered growth characteristics of the progeny virus; first, the virus so passaged could now grow equally well under alkaline and neutral maintenance media, and, secondly, autointerference observable with the parent virus eventually lowered virus yield when neutral maintenance medium was used, but this effect of undiluted passage was eliminated by the use of pH 8.2 maintenance medium.
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PMID:Effect of alkaline maintenance medium upon the growth of rabies virus in chick embryo cells. 23 91

The rabies strain Flury HEP, adapted in our laboratory to BHK21 cells, shows, after 20 passages on this cell, an important modification of its pathogenic characteristics on adult mice, guinea pigs and hamsters. An experimental vaccine made with this adapted strain gives a good sero-conversion rate when injected by the intramuscular route to a group of dogs.
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PMID:[Modification of the strain Flury HEP after its adaptation to cell culture. Development of experimental vaccine for veterinary use]. 26 69

A new lancet with a point length of 1.0 mm suitable for skin prick testing is described. The weal reactions appearing after the use of histamine chloride 1 mg/ml and an extract of Phleum pratense in a 1 HEP concentration (1 histamine equivalent prick) prove suitable as regards size and reproducibility compared with the reactions produced by a more conventional prick test method. The new lancet simplifies the prick test procedure and does not require as much experience as do needles and longer lancets.
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PMID:A new lancet for skin prick testing. 39 Oct 93

The lipid composition of highly purified Flury strain of rabies virus (HEP) propagated in BHK-21 cells in a chemically defined medium was observed to be 6.7% neutral lipids, 15.8% phospholipids, and 1.5% glycolipids. In the virion, phosphatidylethanolamine, phosphatidylcholine, and sphingomyelin were the most abundant phospholipids, accounting for 90% of the total, and the molar ratio of cholesterol to phospholipid was 0.48. Uninfected BHK-21 cell membranes were obtained by nitrogen cavitation techniques and separated by density gradient centrifugation, and the membranes were assayed for purity using 5'-nucleotidase, cytochrome oxidase, and reduced nicotinamide adenine dinucleotide phosphate diaphorase activities. Lipids of the plasma membrane were enriched in cholesterol, phosphatidylcholine, and phosphatidylethanolamine. In contrast, membranes of the endoplasmic reticulum were enriched in phosphatidylcholine, but contained smaller amounts of phosphatidylethanolamine and sphingomyelin. Comparison of the fatty acyl chains of virus and membranes from uninfected cells revealed the virion to have the lowest ratio of C18:1 to C18:0 (1.771), compared with values of about 3.0 for the plasma membrane and endoplasmic reticulum. Total polyenoic fatty acids were enriched in the plasma membrane, whereas the virus contained higher amounts of total saturates than either of the two membrane preparations. Analysis of the polar and neutral lipid fractions as well as the acyl chain analysis suggests the virion has a lipid composition that is intermiediate to that of the plasma membrane and endoplasmic reticulum and is consistent with the view that numerous viral particles are synthesized de novo by not utilizing a preexisting membrane template. From the ratio of cholesterol to phospholipid of 0.48, we calculated that 1.92 X 10(5) molecules of lipid would cover 4.14 X 10(4) nm2 in the form of a bilayer. Considerations of the molecular dimensions of the rabies envelope (total surface area, 5 X 10(4) nm2) as a bilayer suggest that some penetration of lipids by envelope proteins (M and G) is necessary.
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PMID:Lipids of rabies virus and BHK-21 cell membranes. 55 73

In order to elucidate the properties of an inhibitor of rabies virus haemagglutinin in normal animal sera, experiments were made with the HEP Flury strain and calf serum which contains the inhibitor. The results of physico-chemical treatment, gel-filtration and density analysis suggested lipoprotein involvement. When inhibitor and haemagglutinin were mixed, the separate activities could be recovered from the mixture by centrifuging on a sucrose density gradient. By contrast, neither haemagglutinin nor inhibitor could be recovered by this treamtnet when the inhibitor was added at the start of virus growth. The binding of inhibitor with rabies virus during virus growth seems irreversible and different from the binding of inhibitor with pre-formed rabies haemagglutinin.
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PMID:Interaction of non-specific inhibitor and rabies virus haemagglutinim. 56 Apr 27

A purified allergen preparation, Timothy N, from timothy pollen (Phleum pratense) was characterized with respect to chemical properties in comparison with a commercially available crude extract, Timothy O, from the same pollen material. In Timothy N about 94% of the protein and 99% of both hexoses and pentoses were removed during the purification. Timothy N contains a limited number of proteins with molecular weights of about 15,000 and 30,000. A Timothy N solution of 0.010 mg/ml will have an allergenic activity of 1 HEP.
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PMID:A new well-characterized, purified allergen preparation from timothy pollen. I. Chemical properties. 59 Oct 96

The authors verified the following: The FLURY HEP vaccine, with modified virus, confers a higher level of protection when applied by intracerebral route in laboratory animals. With an equal number of viral particles of FLURY HEP modified virus and fixed virus (FUENZALIDA type vaccine), inoculated intracerebrally and intramusculary, a better protection was obtained with the first kind of vaccine, specially by intracerebral route. These conditions suggest the possibility of replication of modified HEP virus in the cells of the central nervous system. The results show that the concentration of neutralizing antibodies in the serum is not the only responsable for protection, but suggests the possibility of factors or of cellular immunity acting in the animal's protection against rabies.
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PMID:[Anti-rabies vaccination. Comparative analysis of the responses of Flury Hep, Fuenzalida, and inactivated Flury Hep vaccines]. 75 69

A very low pathogenic strain of "fixe" rabies (HEP-Flury) gives a rise in mortality in mice immunodepressed with cyclophosphamide. In this experiment model, the rabies antiserum gives efficient protection against the virus.
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PMID:[Effects of cyclophosphamide on rabies infection in the mouse. Protection confered by serotherapy]. 81 5

By measuring the electro-osmotic flow velocity of buffer through a glass capillary, on the inner surface of which cells have been grown, the zota-protential of these cells can be determined without the changes in zota-potential, viability and morphology caused by the chemical, enzymatic or mechanical pretreatment hitherto necessary for cell dispersion in the microelectrophoretic method. Cells grown in a monolayer inside the capillaries were: BGM, HEP-2, and RPMI 1846 (the last one also could be grown in suspension).
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PMID:Zeta-protentials of intact cell monolayers determined by electro-osmosis. 82 49

HEP Flury strain of rabies virus maintained by 7-day chicken egg passage (parent line) and the same strain serially passaged in primary chick embryo (CE) cells using alkaline maintenance medium (AM line) were inoculated to cells of various species. Growth was negative in primary mouse embryo, L and HeLa cells, and positive in primary hamster kidney and BHK21 cells with both lines. An all-or-none difference between the two lines was observed in primary monkey kidney and Vero cells. The parent line did not multiply in these monkey cells, whereas the AM line grew to high titers. In the case of Vero cells a unique cytopathic effect (CPE) was induced by the AM line. After five consecutive passages in Vero cells, the CPE-inducing agent was identified as rabies virus by a neutralization test. It was infective to intracerebrally inoculated suckling mice but not to adult mice, and its Vero cell-infective titer determined by CPE induction was about 1 log lower than the baby mouse-infective and CE plaque-forming titers. In contrast to the AM line, HEP Flury strain receiving 150 CE cell passages under neutral maintenance medium and three other strains receiving similar CE cell passages all failed to grow in Vero cells.
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PMID:Alteration of the in vitro host range of rabies virus after serial chick embryo cell passage using alkaline maintenance medium. 82 85


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