Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.10.1 (ERK)
 95,504 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In this study we describe an in situ hybridization protocol suitable for the measurement of interleukin mRNAs in small numbers of cells in suspension. In this procedure defined numbers of such cells are coated onto defined areas of microscopic slides using LAB-TEK tissue culture chamber slides. 32P-labelled oligonucleotides are then hybridized to them in situ. Binding of the probes is measured by autoradiography using X ray film followed by densitometry (slide blot) or, as in standard in situ protocols, by exposure to photoemulsion. In experiments designed to demonstrate the specificity and feasability of this protocol, peripheral blood T cells were activated with mitogenic stimuli and oligonucleotide probes specific for IL-2, IFN-gamma, or a control probe (IL-2 random) were hybridized to them. Both lymphokine genes were found to be transcribed in two discrete phases with the first peak at 12 h and another peak 48 h after stimulation, as measured semi-quantitatively on a per cell basis using X ray film exposure. The proposed experimental protocol supports the advantages of X ray film autoradiography such as rapidity, simplicity and the objectivity of densitometric evaluation, but also permits microscopic evaluation of individual cells as performed in classical in situ hybridization protocols using photoemulsion.
 ...
PMID:A simple and rapid slide blot method to quantify cytokine-mRNA in small numbers of lymphocytes. 176 64

We evaluated 550 serum samples with four commercially available enzyme immunoassays and Western Blot was used as the confirmatory test for antibodies against human immunodeficiency virus (HIV). The Wellcozyme (Wellcome), Flow HIV-TEK G, and Behring test kits identified all 50 Western Blot positive samples correctly, whereas DuPont failed to detect one sample. None of the kit was able to pickup one sample that showed a faint P24 band on Western Blot strip. The frequency of false positive reaction in the 500 negative serum samples were Wellcome 0%, Behring and DuPont 0.2% and Flow HIV-TEK 0.4%.
 ...
PMID:Detection of HIV-antibody evaluation of four commercially available enzyme immunoassays. 212 70

A micro-enzyme-linked immunosorbent assay (micro-ELISA) using the Salmonella-TEK screen kit was tested for the detection of Salmonella spp. in pure cultures as well as in 30 artificially contaminated food samples and in 45 naturally contaminated food samples. Different raw, fleshy foods and processed foods were used as test products. The artificially contaminated minced meat samples were preenriched in buffered peptone water, and after incubation, different selective enrichment broths were tested. The micro-ELISA optical density values after enrichment and isolation of the different broths were very analogous. The quickest method to detect Salmonella spp. in different foods is to enrich them with Salmosyst broth, which reduces the total analysis time to 31 h. The Salmonella-TEK kit for Salmonella spp. provides a promising test for the detection of Salmonella antigens in food even when they are present at a low concentration (1 to 5 CFU/25 g). The cross-reaction of the anti-Salmonella antibodies, especially to other gram-negative bacteria, is nil.
 ...
PMID:Salmonella-TEK, a rapid screening method for Salmonella species in food. 218 6

Aminoglycoside nephrotoxicosis (AGNT) was induced in ewes by daily SC administration of gentamicin. Changes in urinary indices of renal function during the development of AGNT are reported. Measurements from timed, volume-measured urine samples were made on days 0, 7, and 8 and included creatinine clearance, total excretion (TE) rates of electrolytes (Na, K, Cl, P) and urine volume. Measurements from free-catch urine samples (without volume measurement) were made daily and included fractional excretion (FE) rate of electrolytes, urine osmolality, and urine-to-serum osmolality and urine-to-serum creatinine ratios. With the onset of AGNT, FE rates of Na, K, Cl, and P- increased many fold above baseline values (200x, 4 to 5x, 6 to 9x, and 70 to 95x, respectively, on days 7 and 8), indicating decreased tubular reabsorption or increased tubular secretion. The increased FE rates were not representative of increases in total electrolyte excretion rates. The total excretion of Na (TENa) was mildly increased, TEK was decreased, TECl was unchanged, and TEP was significantly increased on days 7 and 8. Abnormal urinalysis results, glucosuria, and increased FEP preceded appreciable increase in serum creatinine concentration. Other abnormal urinary indices of renal function coincided with or followed the increase in serum creatinine concentration. Urinary indices may help characterize renal function associated with the disease state, but did not provide early indication of AGNT.
 ...
PMID:Urinary indices of renal function in sheep with induced aminoglycoside nephrotoxicosis. 231 20

An autoimmune disease can be the cause of thyroid disfunction. Determination of autoantibodies titers is the best way of demonstrating its existence. We studied 172 thyroid patients (146 females, 26 males) with ages ranging from 15 to 81 years. Thyroid microsomal autoantibodies (TMA) were detected by a modified agglutination test (SERA-TEK kit, Ames Div); a dilution greater than or equal to 1/1600 was considered as diagnostic of autoimmune disease. Patients were classified according to morphological and functional status in 3 groups: GI = non toxic goiter, n = 98 (71 diffuse, 20 multi and 7 uninodular); GII = toxic goiter, n = 62 (52 diffuse, 4 multi, 2 uninodular and 4 subacute thyroiditis); G III = hypothyroidism, n = 12 (5 primary hypothyroidism and 7 chronic thyroiditis). A control group of 30 normal individuals, ages ranging from 19 to 85 years was also studied. Diagnostic titers of TMA were found in 30.8% of group I, 88.5% of group II, 91.6% of group III and only in 6.6% of controls. The high incidence of positive TMA in toxic diffuse goiter (96.1%) as well as in hypothyroid patients was expected since these are typical examples of thyroid autoimmune disease. In the non toxic goiter group, positive TMA were present in 50% of multinodular, 28% of uninodular and 25% of diffuse goiters and the incidence of positive TMA varied according to age, being higher over the age of 40 years and lower under the age of 20 years. We postulate that this unexpected high incidence of positive TMA in non toxic goiter is due to amelioration of chronic iodine deficiency inducing the expression of latent autoimmune disease.(ABSTRACT TRUNCATED AT 250 WORDS)
 ...
PMID:[Thyroid microsomal autoantibodies in thyroid disease: their value as an antigenic marker]. 251 89

A comparative study was carried out to determine the relative sensitivities of eight different cell culture systems to six different herpesviruses of animals. The cells used were: OFL (ovine fetal lung), ML (mink lung), FK (ferret kidney), PTK-2 (potoroo kidney), TEK (turkey embryo kidney), ED (equine dermal), BT (bovine turbinate), and PK15 (porcine kidney). The viruses tested were: PRV (pseudorabies) of swine, CPHV (caprine herpesvirus), IBRV (infectious bovine rhinotracheitis virus), DN-599 strain of bovine herpesvirus type 4, EHV-1 (equine herpesvirus), and CHV (canine herpesvirus). On the basis of virus titers obtained and the time of appearance of CPE (cytopathic effects), ML cells were found to be the most useful because of their sensitivity to all six viruses tested. BT and OFL cells were also found to be highly sensitive to all viruses with the exception of CHV.
 ...
PMID:Propagation and quantitation of animal herpesviruses in eight cell culture systems. 284 31

Total Exchangeable Potassium (TEK) was measured in 31 elderly patients admitted to a geriatric assessment unit. The value in control cases ranged between 26 mEq/kg to 45.5 mEq/kg; the mean values for men and women were 35.5 mEq/kg and 28.4 mEq/kg, respectively. The test was repeated after one week in eight cases, the standard deviation between the two estimations ranged from 0.35 to 1.13 with the exception of one case which was 2.55. Estimations were made in six cases of myxoedema, the initial value was low and on treatment rose by 22.6 per cent to 36.4 per cent which coincided with the clinical improvement.
 ...
PMID:Assessment of potassium metabolism, using 42K, in cases of myxoedema before and after treatment with thyroxine. 445 28

Three passive samplers are now commercially available for NO2. This field validation, conducted in an underground mine, attempted to address both the precision and accuracy of the three now commercially available. The probable sources of NO2 were identified as diesel engines and blasting operations. Comparative sampling was conducted with the passive samplers versus the standard "baseline" impingement method. The three NO2 samplers were as follows: 1) PRO-TEK (DuPont); 2) Palmes (MDA); 3) VaporGard (MSA). Three sets of data consisting of impingers and passive sampler results were taken on top of a moving diesel vehicle over a three-day period. An expanded metal screen was welded in a "free standing" plane above the vehicle to serve as a sampling platform. The evaluation of concentration data suggested that correlations of accuracy and precision versus the impinger method were best for the Palmes and VaporGard samplers. The PRO-TEK sampler does not seem to produce accurate data, but it is somewhat precise. Factors of sensitivity, accuracy, precision, cost, ease of analysis, and stability must be weighed.
 ...
PMID:Field validation study of NO2 personal passive samplers in a "diesel" haulage underground mine. 665 Apr 1

TEK is a newly cloned receptor tyrosine kinase that is expressed predominantly in the endothelium of actively growing blood vessels. Disruption of TEK function in transgenic mice results in a profound defect in vascular development leading to embryonic lethality. These studies show that TEK signaling is indispensable for the development of the embryonic vasculature and suggest that TEK signaling may also be required for the development of the tumor vasculature. Because the ligand for TEK has not been identified, it has been difficult to study signal transduction by this important endothelial receptor. To circumvent this problem, a soluble TEK kinase domain (GTEKH) was developed which could be easily purified, autophosphorylated, and radiolabeled. Using the autophosphorylated, radiolabeled GTEKH to probe a mouse embryo expression library only two candidate signaling molecules were isolated, SH-PTP2 and GRB2. Autophosphorylated GTEKH associated with GRB2 and SH-PTP2 from endothelial lysates and not with PI3 kinase or PLC gamma. The association of GRB2 and SH-PTP2 with TEK was highly dependent on specific tyrosine residues in the TEK c-tail. These studies identify GRB2 and SH-PTP2 as potentially important mediators of TEK signaling that may trigger crucial endothelial responses during embryonic vascular development and during pathologic vascular growth.
 ...
PMID:GRB2 and SH-PTP2: potentially important endothelial signaling molecules downstream of the TEK/TIE2 receptor tyrosine kinase. 747 29

The results, either positive or negative, of the ELISA tests performed on blood donors to detect viral markers, are always validated by reading microplates on photometers. In Rennes, these microplates can be read on MR 7000 (Dynatech) or IEMS (Labsystems) photometers. This step, the last one in the technical sequence, conditions the results. Regular checking of the photometers therefore may enhance the quality of the testing sequence. To check the quality of the photometers, we use a 96-well microplate, the "Universal Calibration Test Plate" marketed by BIO-TEK Instruments USA. The plate includes 8 blank wells, 6 wells equipped with glass filters (3 stained and 3 unstained). This plate comes with a table showing the optical density in relation to the wavelength. We first verify the repeatability of each photometer, and secondly, verify accuracy. The plate also permits verifying the linearity of each photometer. In view of the results, we were able to state that both photometers were accurate, since the results obtained were compatible with an optical density of +/- 0.024 +/- 1%. Do. The tests are repeatable, with a variation coefficient below 0.5%, linearity was verified (r: 0.999 for both photometers). Reading the plate only takes a few minutes and permits daily control of the photometers. It only requires that the test results be reported on a control card to follow-up possible deviations, and technical maintenance can thus be carried out immediately.
 ...
PMID:[Development of a validation method for readers of ELISA microplates]. 776 80


1 2 3 4 5 6 7 8 9 10 Next >>