Gene/Protein
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Drug
Enzyme
Compound
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Target Concepts:
Gene/Protein
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Query: EC:2.7.1.21 (
thymidine kinase
)
7,561
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Methylglyoxal
bis(guanylhydrazone) completely inhibits the induction of
thymidine kinase
after serum stimulation of quiescent fibroblasts only if added within 3 h after serum, whereas calcium deprivation blocks this induction up to 12 h after serum stimulation. Experiments in which one of these blocks was imposed as the other was released confirmed that cells blocked by methylglyoxal bis(guanylhydrazone) are arrested at an earlier stage in G1 than cells blocked by calcium deprivation.
...
PMID:An inhibitor of polyamine synthesis arrests cells at an earlier stage of G1 than does calcium deprivation. 684 51
Biochemical events were investigated in the G1 to S phase progression induced in quiescent rodent cells by human adenovirus type 5 (Ad5) and by serum. Thymidine kinase activity increased after infection of cells with Ad5 or addition of 10% serum. These stimulations were additive. An early viral gene was responsible for induction by Ad5, but the early mutants ts36, ts37, and ts125 induced
thymidine kinase
at the permissive and nonpermissive temperatures. Several differences were found between cells stimulated by serum compared with Ad5. Induction of
thymidine kinase
was delayed in Ad5-infected cells, insensitive to 0.01 microgram/ml actinomycin D and relatively resistant to reduced Ca2+ compared with induction by serum. Ornithine decarboxylase was induced by serum, but not by Ad5, alpha-Methylornithine had little effect on the induction of
thymidine kinase
by Ad5, but reduced the induction of
thymidine kinase
by serum, suggesting that Ad5-induced entry into S phase is uncoupled from polyamine biosynthesis.
Methylglyoxal
bis(guanylhydrazone), however, prevented the induction of
thymidine kinase
by both serum and Ad5. Adenovirus infection appears to induce cellular DNA synthesis and
thymidine kinase
in G1-arrested cells by a mechanism different from serum, and bypasses events in the normal G1 to S phase progression.
...
PMID:A biochemical investigation of the adenovirus-induced G1 to S phase progression: thymidine kinase, ornithine decarboxylase, and inhibitors of polyamine biosynthesis. 706 69
