Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.1.21 (
thymidine kinase
)
7,561
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We present evidence that
CRE-BP1
binding to the cyclic AMP (cAMP) response element (CRE) is a transcriptional activator. Transcriptional activation was assayed by cotransfection into CV-1 cells of a
CRE-BP1
expression plasmid together with a reporter plasmid in which the
thymidine kinase
promoter and four tandem repeats of CRE were linked to the chloramphenicol acetyltransferase (CAT) gene. Cotransfection with the
CRE-BP1
expression plasmid caused an 8-fold stimulation of CAT activity, while cotransfection with the plasmids to express
CRE-BP1
and c-Jun induced a 32-fold stimulation of CAT activity, suggesting that a heterodimer of
CRE-BP1
with c-Jun is a stronger trans-activator than a homodimer of
CRE-BP1
. By using a series of deletion and point mutants of
CRE-BP1
in this cotransfection assay, two functional domains of
CRE-BP1
were identified: the putative metal finger structure in the amino-terminal region and the leucine zipper motif linked to a cluster of basic amino acids in the carboxyl-terminal region. The former was a transcriptional activation domain in the absence of c-Jun. The latter was a DNA-binding domain, and was essential in both the presence and absence of c-Jun.
...
PMID:Identification of the functional domains of the transcriptional regulator CRE-BP1. 183 93
