Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.7.1.1 (hexokinase)
 5,274 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A method for producing large quantities of pure proximal tubule cells is described. The procedure involves collagenase perfusion to prepare kidney cells that are separated by centrifugal elutriation to produce a purified cell preparation of proximal tubule cells (PCS). The elutriation-purified cells were treated with a rabbit polyclonal antibody to rat gamma-glutamyl transpeptidase (GGT). The resulting antibody-labelled cells were then incubated with Dynabeads coated with a sheep anti-rabbit IgG antibody. The Dynabeads, which are monodispersed polystyrene beads with a magnetic ferrite core, bind specifically to the antibody-labelled cells. The cell-bead complexes were then harvested with a magnet and washed twice to remove cells not labelled with antibody. The procedure is simple and rapid, and can be used to produce 20-25 x 10(6) cells. The cells exhibit a well defined brush border membrane with increased GGT and alkaline phosphatase enzyme activities. Concomitant with this is a four-fold decrease in hexokinase activity, which demonstrates that contaminating distal and loop of Henle tubule cells have been removed.
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PMID:Immunomagnetic purification of rat proximal kidney cells. 2069 84

Arsenic is an environmental pollutant and its contamination in drinking water poses serious world wide environmental health threats. It produces multiple adverse effects in various tissues, including the kidney. However, biochemical mechanism and renal response to its toxic insult are not completely elucidated. We hypothesized that sodium arsenate (ARS) induces oxidative stress and alters the structure and metabolic functions of kidney. Male Wistar rats were administered ARS (10 mg/kg body weight/day), intraperitoneally daily for 10 days. ARS administration increased blood urea nitrogen, serum creatinine, cholesterol, glucose, and phospholipids but decreased inorganic phosphate, indicating kidney toxicity. The activity of brush border membrane (BBM) enzymes significantly lowered in both cortex and medulla. Activity of hexokinase, lactate dehydrogenase, glucose-6-phosphate dehydrogenases, and NADP-malic enzyme significantly increased whereas malate dehydrogenase, glucose-6-phosphatase, and fructose 1,6 bis phosphatase decreased by ARS exposure. The activity of superoxide dismutase, GSH-peroxidase, and catalase were selectively altered in renal tissues along with an increase in lipid peroxidation. The present results indicated that ARS induced oxidative stress caused severe renal damage that resulted in altered levels of carbohydrate metabolism and BBM enzymes.
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PMID:Studies on the effect of sodium arsenate on the enzymes of carbohydrate metabolism, brush border membrane, and oxidative stress in the rat kidney. 2456 57

Hexokinase domain component 1 (HKDC1) is a recently discovered novel protein, which is being promoted as a putative fifth hexokinase. Although the exact role HKDC1 plays in physiology is still unclear, it has been shown to be important during pregnancy in the regulation of glucose homeostasis. In this study, we have comprehensively studied the expression pattern of HKDC1 in the human body. Using human tissue sample, immunohistochemistry imaging was performed. Our studies indicate that the tissues with highest HKDC1 expression were the brush border epithelium of the intestines, parts of the pancreas, and lung alveolar macrophages. Future directions will be to understand the role of this fifth hexokinase in these tissues, with a focus on its relative function as compared with other endogenously expressed hexokinases.
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PMID:Studies on the Tissue Localization of HKDC1, a Putative Novel Fifth Hexokinase, in Humans. 2940 4


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