Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.7.1.1 (hexokinase)
 5,274 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Aldehyde oxidase (Ao) of Anopheles albimanus Wiedemann was mapped on chromosome 3. The sequence is hexokinase-1--19.2 +/- 1.8--stripe--28.3 +/- 2.2--beta-hydroxy acid dehydrogenase--3.6 +/- 0.3--aldehyde oxidase--2.6 +/- 0.4--esterase-8--6.1 +/- 1.9--esterase-4--?--esterase-6 (phosphoglucomutase). Aldehyde oxidase is 26.1 +/- 2.5 from phosphoglucomutase and 27.2 +/- 1.6 from esterase-6. The one-band electromorph of Ao in homozygotes and the three-band type in heterozygotes suggest that the enzyme is a dimer. The isoelectric points of slow and fast allozymes are 5.5 and 4.8, respectively. A variety of electrophoretic techniques was used to determine if the allozymes of Ao can be differentiated on a basis other than mobility. The slow, fast, and hybrid genotypes were analyzed for differences in thermostability, reactivity to thiol reagent, susceptibility to urea denaturation, substrate specificities, and response to chelating agents. The relative effect of p]H on allozymes was tested by varying the pH of the staining buffer over a range of 4-12. No significant differences were detected among allozymes and no additional allelic variations were observed.
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PMID:Genetic mapping and characterization of aldehyde oxidase of Anopheles albimanus (Diptera: Culicidae). 662 39

beta-Hydroxy acid dehydrogenase (beta-Had-2) of Anopheles albimanus was assigned to chromosome 3. The apparent sequence of loci on chromosome 3 is hexokinase-1--22--stripe--28--beta-hydroxy acid dehydrogenase-2--4--aldehyde oxidase--2--esterase-8--4--esterase-4--?--phosphoglucomutase--?--esterase-6. beta-Hydroxy acid dehydrogenase is 25 and 30 map units from phosphoglucomutase and esterase-6, respectively. The one-band electromorph of beta-Had-2 in homozygotes and the three-band type in heterozygotes suggest that the enzyme is a dimer. A variety of electrophoretic techniques and spectrophotometric analysis were used to determine if the allozymes of beta-Had-2 can be differentiated on a basis other than mobility. No differences were detected among the allozymes on the basis of thermostability, urea denaturation, response to thiol reagents, chelating agents, or changes in coenzyme and substrate concentrations. No heterogeneity within allozymes separated by electrophoresis was detected by using thermostability tests.
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PMID:Genetic and physiochemical studies on beta-hydroxy acid dehydrogenase in Anopheles albimanus. 666 Nov 76