Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.1.1 (
hexokinase
)
5,274
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Most biological fluids contain both neutral and
acid alpha-glucosidase
. Optimal conditions were therefore developed for the selective determination of the activity of neutral and
acid alpha-glucosidase
, using 2-step, discontinuous assays. In the first step of the assay of neutral alpha-glucosidase, glucose was liberated from maltose (citrate-phosphate buffer, pH 6.8, 20 mmol/l maltose, 25 mmol/l turanose). Under these incubation conditions, turanose inhibited the residual activity of
acid alpha-glucosidase
almost completely without influencing the activity of neutral alpha-glucosidase. In the first step of the
acid alpha-glucosidase
assay, glucose was liberated from maltose (citrate-phosphate buffer, pH 3.8, 50 mmol/l maltose, 2 mol/l potassium chloride). Under these incubation conditions, potassium ions stimulate the activity of
acid alpha-glucosidase
and simultaneously inhibit almost completely the residual activity of neutral alpha-glucosidase. In the second step of the assay of neutral and
acid alpha-glucosidase
, the liberated glucose was measured by
hexokinase
/glucose-6-phosphate dehydrogenase. The effect of turanose and potassium ions on neutral and
acid alpha-glucosidase
from human urine was characterized.
...
PMID:Selective determination of the activities of neutral and acid alpha-glucosidase using discontinuous assays. 635 65
