Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.7.1.1 (hexokinase)
 5,274 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

This collaborative study on the determination of glucose and fructose in wine was performed by 18 laboratories on 4 matched pairs of commercial wine. The method uses the enzymes hexokinase, glucose-6-phosphate dehydrogenase, and phosphoglucose isomerase and the coenzyme nicotinamide-adenine dinucleotide phosphate. Both glucose and fructose can be determined in the same sample without separation. The method is simple but care is necessary to ensure precise transfer of small volumes. Repeatability and reproducibility standard deviations for glucose ranged from 2.6 to 14.6 mg/L and 4.7 to 16.5 mg/L, respectively. Repeatability and reproducibility values for fructose ranged from 2.4 to 16.1 mg/L and 6.0 to 21.3 mg/L, respectively. The method has been adopted official first action.
J Assoc Off Anal Chem
PMID:Enzymatic-ultraviolet determination of glucose and fructose in wine: collaborative study. 405 18