Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.1.1 (hexokinase)
 5,274 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Resting and stimulated fluxes of sodium and potassium across the giant axon of the marine annelid, Myxicola infundibulum, have been characterized using the technique of internal dialysis. In most respects the ion movements were found to be similar to those in squid axons. Sodium efflux and potassium influx were found to be active, cardiac glycoside-sensitive fluxes, with a variable coupling ratio. However, when [ATP]i was lowered to less than 20 microM by treatment with cyanide and continuous dialysis, or to less than 2 microM by dialysis with glucose following injection of hexokinase, Na efflux and K influx were unaltered. The maintained fluxes were not accounted for by an increased passive permeability of the axolemma, although 30-60% of the Na efflux appeared to be due to Na-Na exchange. An altered form of Na pump operation at low [ATP]i is a more likely explanation than an alternate energy source, or an ATP source proximate to the axolemma. The transient response of 22Na efflux to a change in [22Na]i was found to be much slower than in squid, tau = 360 sec. The efflux delay could only be accounted for by an extra-axonal diffusion barrier, which is probably the basement membrane surrounding the ventral nerve cord.
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PMID:Sodium and potassium fluxes across the dialyzed giant axon of Myxicola. 31 30

This study determined how selected functional, metabolic, and contractile properties were impacted by sodium pivalate, a compound which creates a secondary carnitine deficiency. Young male rats received either sodium pivalate (20 mM, PIV) or sodium bicarbonate (20 mM, CONTR) in their drinking water. After 11-12 weeks cardiac function and glucose oxidation rates were measured in isolated, perfused working heart preparations. Hearts were also analyzed for carnitine content, activities of hexokinase (HK), citrate synthase (CS), and B-hydroxyacyl CoA dehydrogenase (HOAD), and myosin isoenzyme distribution. Sodium pivalate treatment significantly reduced cardiac carnitine content and increased glucose oxidation but did not alter cardiac functional capacity. HK activity was increased in the PIV group (p < 0.05), and HOAD activity decreased (p < 0.05). CS activity and myosin isoform distribution (VI > 85%) remained unchanged. These results demonstrate that pivalate treatment of this duration and the accompanying carnitine deficiency shift cardiac substrate utilization without compromising cardiac functional capacity.
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PMID:Sodium pivalate reduces cardiac carnitine content and increases glucose oxidation without affecting cardiac functional capacity. 747 77

Trigonella foenum graecum seed powder (TSP) and Sodium Orthovanadate (SOV) have been shown to demonstrate antidiabetic effects by stabilizing glucose homeostasis and carbohydrate metabolism in experimental type-1 diabetes. However their efficacy in controlling histopathological and biochemical abnormalities in ocular tissues associated with diabetic retinopathy is not known. The purpose of this study was to investigate the comparative efficacy of individual as well as combination therapy of TSP and SOV in 8 weeks diabetic rat lens and retina. Retinas and lenses were taken from control, alloxan-induced diabetic rats and diabetic rats treated separately with insulin, 5%TSP, SOV (0.6 mg/ml) and a combined dose of SOV (0.2 mg/ml) and 5%TSP for 60 days. Control and each experimental group had six rats. Alterations in the activities of enzymes HK (hexokinase), AR (aldose reductase), SDH (sorbitol dehydrogenase), G-6-PD (glucose-6-phosphate dehydrogenase), GPx (glutathione peroxidase), GR (glutathione reductase) and levels of metabolites like sorbitol, fructose, glucose, MDA (malondialdehyde) and GSH (reduced glutathione) were measured in the cytosolic fraction of lenses besides measuring blood glucose levels and glycosylated haemoglobin. Histopathological abnormalities were studied in the lens using photomicrography and retina using transmission electron microscopy. Blood glucose, glycosylated haemoglobin levels and polyol pathway enzymes AR and SDH increased significantly causing accumulation of sorbitol and fructose in the diabetic lens and treatment with SOV and TSP significantly (p < 0.05) decreased these to control levels. Similarly, SOV and TSP treatments modulated the activities of HK, G-6-PD, GPx and GR in the rat lens to control values. Ultrastructure of the diabetic retina revealed disintegration of the inner nuclear layer cells with reduction in rough endoplasmic reticulum and swelling of mitochondria in the bipolar cells; and these histopathological events were effectively restored to control state by SOV and TSP treatments. In this study SOV and TSP effectively controlled ocular histopathological and biochemical abnormalities associated with experimental type-1 diabetes, and a combination regimen of low dose of SOV with TSP demonstrated the most significant effect. In conclusion, the potential of SOV and TSP alone or in low dose combination may be considered as promising approaches for the prevention of diabetic retinopathy and other ocular disorders.
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PMID:Long-term effect of Trigonella foenum graecum and its combination with sodium orthovanadate in preventing histopathological and biochemical abnormalities in diabetic rat ocular tissues. 1671 75

A salt-sensitive genotype of Solanum lycopersicum cv. Volgogradskij was submitted to a 6-day treatment with high salt (100, 200 mM NaCl), allowed to recover for 6 days and then submitted to a second period of salt stress in order to study changes in carbohydrate metabolism related to salt adaptation. The ion, soluble sugar and starch contents, as well as sucrose biosynthetic and sugar mobilizing enzyme activities and transcript levels were determined during the salt stress/recovery/stress cycle. Sodium ions were found to accumulate preferentially in old leaves. Young leaves accumulated lower levels of sodium ions but maintained control levels of potassium ions. Hexoses accumulated to higher levels and starch was better maintained in young compared to old leaves during the two salt treatments. Sucrose accumulated dramatically only in old leaves during the initial salt treatment. Sugar accumulation was not related to decreases in the activities of sugar mobilizing enzymes, acid (EC 3.2.1.25) and neutral (EC 3.2.1.26) invertases, sucrose synthase (EC 2.4.1.13) and hexokinase (EC 2.7.1.1). The activity of the biosynthetic enzyme sucrose phosphate synthase (EC 2.3.1.14) was linked to changes in sucrose levels but not with transcript levels. These results point to the importance of post-transcriptional regulation. Transcriptional regulation could nevertheless be seen in the down-regulation of ribulose bisphosphate carboxylase small subunit (EC 4.1.1.39) in old compared to young leaves, but this was not related to sugar levels.
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PMID:Adaptive response to salt involving carbohydrate metabolism in leaves of a salt-sensitive tomato cultivar. 1762 95