Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.7.1.1 (hexokinase)
 5,274 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An earlier graph theoretical model of metabolic and gene-expression networks has been modified and extended to include the effect of electrical potentials on binding constants, representation of uncatalyzed processes, and treatment of parallel reactions catalyzed by a single enzyme. Formal operations on the graph, which are facilitated by a set of standardized guidelines, identify the feedback signals in the network and rank them according to their influence. The technique was applied to a model of glycolysis in ascites tumor cells in the absence and presence of 12.5 mM exogenous glucose. Feedback regulation was widely distributed and mostly due to binding of adenine nucleotide cofactors to the enzymes of the network. The major changes in feedback regulation on adding glucose is the relief of inhibition of hexokinase and phosphofructokinase and the activation of pyruvate kinase. We conclude that regulation of tumor cell glycolysis is not restricted to hexokinase or to (Na+,K+)-ATPase as was previously suggested by others.
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PMID:Identification of regulatory properties of metabolic networks by graph theoretical modeling. 189 Aug 46

The aim of this study was the evaluation of the effects of a subchronic exposure to malathion, an organophosphorus (OP) insecticide, on plasma glucose and hepatic enzymes of glycogenolysis and glycolysis in rats in vivo. Malathion was administered intragastrically by stomach tube in the amount of 1 ml corn oil containing 100mg/kg body weight (BW) daily for 32 days. At the end of the experiment, the liver was removed. The activities of glycogen phosphorylase (GP) and hexokinase (HK) were analysed in the homogenate. The methodology employed was a non-denaturing electrophoresis followed by activity-staining (native PAGE). Malathion decrease GP activity by 50% and increase HK activity by 10%. In addition, an hepatomegaly was recorded with a rise in the hepatic glycogen rate in malathion-treated rats. Moreover, subchronic administration of malathion has no effect on blood glucose concentration. The storage of glycogen in liver may be due to a stimulation of insulin secretion after the inhibition of acethylcholinesterase activity in pancreatic beta cells by malathion. These findings were in favour of an activation of glycogen storage by malathion.
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PMID:Effect of subchronic exposure to malathion on glycogen phosphorylase and hexokinase activities in rat liver using native PAGE. 1662 Dec 13

Regarding the widespread use of organophosphorous pesticides (OP) especially malathion in environment and reported cases of muscle disturbances in human and animal, the present work was undertaken to explore effects of malathion subchronic exposure on rat leg skeletal muscle glucose metabolism by measuring key enzymes of glycogenolysis and glycolysis. Malathion was administered through food for 4 weeks at concentrations of 100, 200, and 400ppm to rats. Activities of enzymes including glycogen phosphorylase (GP), hexokinase (HK), and phosphofructokinase-1 (PFK) were measured in skeletal muscle homogenate of exposed rats. Levels of glucose and insulin were measured in blood. Four weeks administration of malathion at doses of 200 and 400ppm increased blood glucose concentrations to 44.4 and 60.6% of control, respectively. Malathion at doses of 200 and 400ppm increased blood insulin concentration to 36.6 and 143.2% of control, respectively. Malathion at doses of 100, 200, and 400ppm increased muscle PFK activity to 40.4, 53.5, and 83.1% of control, respectively. Malathion at doses of 400ppm increased skeletal muscle GP to 91.6% of control. Skeletal muscle HK was not influenced by malathion treatment. It is concluded that malathion influences muscle glycogenolysis and glycolysis as well as secretion of insulin from pancreas which all may explain diabetic potential of malathion.
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PMID:Effects of malathion subchronic exposure on rat skeletal muscle glucose metabolism. 2178 76