Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.7.1.1 (
hexokinase
)
5,274
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A new continuous spectrophotometric assay is demonstrated for Escherichia coli
alanyl-tRNA synthetase
. It involves beta-gamma adenylyl imidophosphate as a substitute for ATP in the pyrophosphate exchange reaction. The net conversion of beta-gamma adenylyl imidophosphate to ATP can be linked to NADP reduction by
hexokinase
and glucose-6-P dehydrogenase catalyzed reactions, which can be monitored at 340 nm. This assay can be extended to other aminoacyl-tRNA synthetases which can use beta-gamma nonhydrolyzable analogs of ATP as an ATP substitute.
...
PMID:A continuous spectrophotometric assay for Escherichia coli alanyl-transfer RNA synthetase. 635 84
