Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
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Gene/Protein
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Target Concepts:
Gene/Protein
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Query: EC:2.7.1.1 (
hexokinase
)
5,274
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The autodiploid strain of Pullularia pullulans 1125(4)(13) that differs from the haploid strain 1125(4) in the rate of synthesis of the polysaccharide pullulan has a higher activity of
hexokinase
in cell-free preparations. A change in the activity of
hexokinase
in the diploid strain coincides in time with a change in the rate of the polysaccharide synthesis: the activity of
hexokinase
in cell-free preparations is highest when the rate of pullulan synthesis is maximal. In the haploid strain, the activity of
hexokinase
in preparations from the cultures grown for 24, 38 and 48 hours remains at the same level, and then decreases by the 72nd hour. The activity of
sucrose phosphorylase
in cell-free preparations of the haploid strain increases with aging of the culture; in preparations of the diploid strain, the activity decreases by the 38th hour and then increases like in the haploid strain. These data make it possible to suggest that the activity of
hexokinase
, rather than that of
sucrose phosphorylase
, influences the rate of pullulan synthesis.
...
PMID:[Hexokinase and sucrose phosphorylase activity of cell-free preparations of haploid and diploid strains of Pullularia pullulans]. 644 59
beta-Fructofuranosidase, alpha-glucosidase, beta-glucosidase, alpha-mannosidase, beta-mannosidase,
sucrose phosphorylase
, glucosyltransferase and fructosyltransferase were separated by isoelectric focusing and sensitively detected to be slightly diffuse and insoluble spots in thin-layer gels, supported by a glass plate, by release of monosugars or a sugar phosphate, followed by conversion to glucose-6-phosphate (G6P) and then by reduction of NADP+ to NADPH, terminated by the formation of reduced Nitroblue Tetrazolium (NBT). Approximately 1-10 mU of enzyme was focused and the gel, after washing with a buffer, was partially dried and directly stained by uniformly spreading on the gel surface a staining medium containing sucrose or nitrophenyl glycosides as substrates, intermediary enzymes such as
hexokinase
, mutase and/or isomerase, NADP+, ATP, Mg+, phenazine methosulfate (PMS) and NBT. Specific staining procedures for each of these activities, on sucrose or on the glycosides as substrates, and staining procedures for multiple activities are described, with the conditions necessary for optimal development.
...
PMID:Glucose, fructose, mannose and/or glucose-1-phosphate-releasing activity stains for glycosidases and glycosyltransferases in gels after isoelectric focusing. 751 61
