Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.7.1.1 (hexokinase)
 5,274 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An automated kinetic assay for the determination of glucose in blood is described. The method employs the enzyme glucose dehydrogenase in the presence of mutarotase, with nicotinamide adenine dinucleotide as hydrogen acceptor. The analytical parameters of the method are determined and the flexibility of the method in relation to sample volume and sensitivity is discussed. Finally, the method is compared with automated glucose oxidase and hexokinase procedures.
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PMID:A rapid kinetic assay for glucose using glucose dehydrogenase. 3 97

The pathway of glucose metabolism in Pseudomonas aeruginosa was regulated by the availability of glucose and related compounds. On changing from an ammonium limitation to a glucose limitation, the organism responded by adjusting its metabolism substantially from the extracellular direct oxidative pathway to the intracellular phosphorylative route. This change was achieved by repression of the transport systems for gluconate and 2-oxogluconate and of the associated enzymes for 2-oxogluconate metabolism and gluconate kinase, while increasing the levels of glucose transport, hexokinase and glucose 6-phosphate dehydrogenase. The role of gluconate, produced by the action of glucose dehydrogenase, as a major inhibitory factor for glucose transport, and the possible significance of these regulatory mechanisms to the organism in its natural environment, are discussed.
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PMID:The role of glucose limitation in the regulation of the transport of glucose, gluconate and 2-oxogluconate, and of glucose metabolism in Pseudomonas aeruginosa. 17 10

In 28 dogs the distal articular cartilage of the femur was removed and the regenerating articular surface on the 70th postoperative day was studied histochemically for hexokinase, glucose-6-phosphatase, phosphohexose-isomerase, fructose-1, 6-diphosphatase, aldolase, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase, lactate dehydrogenase isoenzymes, phosphoglucomutase, phosphorylase, glycogen synthetase, UDP--glucose dehydrogenase, and UDP-glucuronic acid-4-epimerase. The articular surface consisted of fibrous tissue and of cartilage islets. The latter contained cells differentiating into cartilage and young chondrocytes. The glycolytic enzymes reacted positively in the regenerative articular surface. Enzyme activities were higher in the cells (particularly the chondroblasts and young chondrocytes) of the cartilage islets than in the connective tissue. In the cells differentiations into cartilage, beside the LDH isoenzymes characteristic of glycolysis, a significant LDH1 and LDH2 activity was observed. At the same site the presence of fructose-1, 6-diphosphatase-activity could be assumed, but there was no glucose-6-phosphatase activity. Glycogen synthesis proceeded in the cells of the cartilage islets and UDP-glucuronic acid-4-epimerase activity was observed in the differentiated cells. UDP-glucose dehydrogenase activity was positive in every section of the articular surface.
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PMID:Studies on cartilage formation. XX. Histochemical investigation of some enzymes of glycogen metabolsim in regenerative articular surfaces. 18 10

We report a method for immobilizing glucose dehydrogenase on the inside surface of nylon tubes to produce an immobilized-enzyme nylon-tube reactor. The glucose dehydrogenase reactor is integrated into the flow system of a continuous-flow analyzer to facilitate routine analysis of serum glucose at 50 samples/h. We compared results with those by the reference hexokinase/glucose-6-phosphate dehydrogenase solution method. The coefficient of correlation was r = 0.996. A glucose dehydrogenase reactor made starting with 1 mg (250 U) of enzyme was stable during eight weeks of continuous use, that is, for nearly 3500 tests. This reduced the cost of the assay by at least 50-fold, compared with that for a commercial glucose dehydrogenase test pack method.
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PMID:Routine glucose determination in serum by use of an immobilized glucose dehydrogenase nylon-tube reactor. 45 81

A micromethod for the determination of glucose in 20 microliter of capillary blood using glucose dehydrogenase is described. After deproteinisation with uranyl acetate, the samples are analysed by an Autoanalyzer II method or by a manual procedure. Precision and accuracy are well correlated with the hexokinase-glucose-6-phosphate dehydrogenase method. Eleven months experience have shown the practicability and economic advantages of this method.
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PMID:[Microdetermination of glucose using glucose dehydrogenase, with independent sample preparation in the routine laboratory (author's transl)]. 64 49

The determination of capillary blood glucose after deproteinization using the aca is described. The method, which uses the hexokinase/glucose-6-phosphate dehydrogenase reaction, is compared with the glucose dehydrogenase method. The comparison shows that glucose values measured in capillary blood are essentially the same in both methods. The requirements for quality control are fulfilled. The method is not influenced by hemolysis, bilirubinemia, and hypertriglyceridemia.
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PMID:[The determination of capillary blood glucose using the automatic clinical analyzer (aca) Dupont (author's transl)]. 64 50

The method of estimation of glucose described by Trinder was adapted to analysis by centrifugation (Centrifichem). A kinetic study of drug interferences (ascorbic acid, gentisic acid, levodopa, alphamethyldopa) was carried out. The choice of parameters of the method was justified by an increased specificity of the reaction and by an extremely narrow correlation with the technics using hexokinase (r = 0.994) and glucose dehydrogenase (r = 0.996).
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PMID:[Adaptation to the Centrifichem of the estimation of blood sugar according to Trinder's method. Kinetic study of the main drug interferences (author's transl)]. 69 31

The hexokinase method and a method using glucose dehydrogenase are compared. In general, the two methods show good agreement. The samples were not deproteinized and were obtained from fasting patients of the medical, surgical, rheumatological and hemodialysis clinics. The possible influence of anticoagulants, preservatives and hemoglobin on the determination of glucose with glucose dehydrogenase was checked; there was no evidence of interference. Among the carbohydrates which were tested, only 2-deoxyglucose gave a positive reaction. In addition, this is a reliable and cheap method. The multiplication factor for the photometric analysis remains stable for seven days with a linearity between 0.55 and 44.4 mmol/1 glucose.
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PMID:[Investigation of a new method for the determination of glucose, using the Greiner Selective Analyzer II (GSA II) (author's transl)]. 85 3

A manual version and mechanized versions for several types of analyzers are described for the kinetic determination of glucose with glucose dehydrogenase. Results of glucose determinations with and without deproteinization of samples are discussed. The correlation of results with the hexokinase method is shown.
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PMID:Kinetic determination of glucose concentrations with glucose dehydrogenase. 86 83

A stirrer containing immobilized glucose dehydrogenase has been successfully used for determining glucose in plasma. The device is usable for at least two months and for about 500 assays. The reaction was measured kinetically and linearity was observed to 4 g of glucose per liter. Tested with aqueous glucose and with deproteinized plasma, within-day and day-to-day precision were good. Interference and method-comparison (hexokinase method) were examined. The performance of this system makes the technique useful and attractive for routine use in small-volume clinical laboratories.
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PMID:Determination of plasma glucose with use of a stirrer containing immobilized glucose dehydrogenase. 87 Feb 54


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