Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.7.1.1 (hexokinase)
 5,274 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Hyperinsulinemia was produced in fetal rhesus monkeys for 21 days in the last third of gestation by subcutaneous pork insulin injected at 19 U a day. Plasma insulin concentrations in treated fetuses (N = 4) were 3525 microU/ml. There was no difference in paired pre- and post-treatment fetal plasma glucose concentration. Activity of the hepatic enzymes that promote glucose utilization (glucokinase and hexokinase) and glycolysis (phosphofructokinase, pyruvate kinase, and pyruvate dehydrogenase) was unaffected. Similarly, glycogen metabolism enzymes (active and inactive synthase and phosphorylase) were unaltered. Two gluconeogenic enzymes (PEPCK and glucose-6-phosphatase) were diminished in the treated group compared with controls. Fetal hyperinsulinemia enhanced lipogenic and NADPH-producing enzyme activities, as evidenced by a twofold increase in fatty acid synthase and in citrate cleavage enzyme activity. Malic enzyme was absent. Hyperinsulinemia with euglycemia (1) increases the activity of enzymes that participate in lipogenesis, (2) decreases some of those controlling gluconeogenesis, and (3) has no effect on the enzymes of glycolysis.
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PMID:Chronic hyperinsulinemia in the fetal rhesus monkey: effects on hepatic enzymes active in lipogenesis and carbohydrate metabolism. 22 50

Interpretation of enzymatic data requires consideration of the food intake of each animal studied. Food intake and body mass gain are closely correlated in rapidly growing animals. Direct measurement of food intake by individual fish within a school is nearly impossible. We examined the relationship between growth and liver enzyme activity as a means of inferring the food intake of individual fish within a school. Trout, identified by passive integrated transponder implants, were fed either 0, 0.3, 1, or 2% body mass/d to produce a wide range of growth rates. The activities of five enzymes, predominantly localized in liver, were measured. Results showed that, although the magnitude of response differed, increases in total liver activities of all five enzymes measured were linearly related to growth. Hexokinase (EC 2.7.1.1) increased at a rate below, and beta-D-glucose:NAD(P)+1-oxidoreductase (EC 1.1.1.47) increased at a rate equivalent to, observed increases in total liver mass. Malic enzyme (EC 1.1.1.40), glucose-6-phosphate dehydrogenase (EC 1.1.1.49) and 6-phosphogluconate dehydrogenase (EC 1.1.1.44) showed preferential increases in activity as food intake increased. Correlation of enzyme activities measured in fish fed restricted rations with either growth or nominal feeding rate showed that growth of individual fish was more closely related to liver enzyme activities than nominal feeding rate.
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PMID:Relationship between growth and selected liver enzyme activities of individual rainbow trout. 205 Dec 29

Abnormal glucose and lipid metabolism in striated muscles and arterial wall has been demonstrated in 3 species: the pig, the dog, and human Type 2 diabetic patients, sharing the common feature of peripheral hyperinsulinaemia. In this study eighteen consecutive patients undergoing coronary bypass surgery and eight control patients were examined. Prior to surgery an oral glucose tolerance test showed that eleven out of eighteen patients had impaired glucose tolerance and significantly elevated fasting immune reactive insulin (IRI) and C-peptide concentrations. There was a statistically significant correlation between the 2 hour blood glucose value and the fasting plasma insulin level (R = 0.55, p less than 0.05). During the operation, aortic and muscle biopsies were taken. The eighteen patients undergoing coronary bypass surgery showed disturbances in glucose metabolism, i.e. decreased activity of glycolytic enzymes (hexokinase 0.30 +/- 0.06 versus 0.40 +/- 0.06 U/g, p less than 0.001, and phosphofructokinase 0.48 +/- 0.09 versus 0.61 +/- 0.07 U/g, p less than 0.01). Malic enzyme activity was increased in all patients (0.17 +/- 0.03 versus 0.06 +/- 0.02 U/g, p less than 0.001). Glucose-6-phosphate dehydrogenase was increased in the eleven patients with impaired glucose tolerance (0.55 +/- 0.10 versus 0.30 +/- 0.07, p less than 0.01) parallel to a significant increase in triglyceride content in the aortic wall (16.1 +/- 4.8 versus 3.7 +/- 3.2 mumol/g, p less than 0.01) as well as in the striated muscles (374 +/- 44 versus 48 +/- 6 mumol/g, p less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Intracellular metabolism in biopsies from the aorta in patients undergoing coronary bypass surgery. 295 78

The activities of various ammoniagenic, gluconeogenic, and glycolytic enzymes were measured in the renal cortex and also in the liver of rats made diabetic with streptozotocin. Five groups of animals were studied: normal, normoglycemic diabetic (insulin therapy), hyperglycemic, ketoacidotic, and ammonium chloride treated rats. Glutaminase I, glutamate dehydrogenase, glutamine synthetase, phosphoenolpyruvate carboxykinase (PEPCK), hexokinase, phosphofructokinase, fructose-1,6-diphosphatase, malate dehydrogenase, malic enzyme, and lactate dehydrogenase were measured. Renal glutaminase I activity rose during ketoacidosis and ammonium chloride acidosis. Glutamate dehydrogenase in the kidney rose only in ammonium chloride treated animals. Glutamine synthetase showed no particular variation. PEPCK rose in diabetic hyperglycemic animals and more so during ketoacidosis and ammonium chloride acidosis. It also rose in the liver of the diabetic animals. Hexokinase activity in the kidney rose in diabetic insulin-treated normoglycemic rats and also during ketoacidosis. The same pattern was observed in the liver of these diabetic rats. Renal and hepatic phosphofructokinase activities were elevated in all groups of experimental animals. Fructose-1,6-diphosphatase and malate dehydrogenase did not vary significantly in the kidney and the liver. Malic enzyme was lower in the kidney and liver of the hyperglycemic diabetic animals and also in the liver of the ketoacidotic rats. Lactate dehydrogenase fell slightly in the liver of diabetic hyperglycemic and NH4Cl acidotic animals. The present study indicates that glutaminase I is associated with the first step of increased renal ammoniagenesis during ketoacidosis. PEPCK activity is influenced both by hyperglycemia and ketoacidosis, acidosis playing an additional role. Insulin appears to prevent renal gluconeogenesis and to favour glycolysis. The latter would seem to remain operative in hyperglycemic and ketoacidotic diabetic animals.
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PMID:Renal enzymes during experimental diabetes mellitus in the rat. Role of insulin, carbohydrate metabolism, and ketoacidosis. 623 75

The influence of two carbohydrates, starch and glucose, on hepatic enzyme activities of juvenile tilapia,Oreochromis niloticus xO. aureus, were investigated. Fish were fed either starch or glucose diet solely for 12 weeks or fed either starch or glucose diet for the first 6 weeks and then switched the diet each other for the last 6 weeks. Fish fed only the starch diet gained more (p < 0.05) body weight, had better feed conversion, higher protein deposition and energy retention values than fish fed the glucose diet. These parameters generally decreased in fish when the starch diet was fed first and then switched to the glucose diet, or increased in fish when the glucose diet was fed first and then switched to the starch diet. The starch - fed fish had higher body lipid content than glucose - fed fish. Body lipid decreased in fish transferred from the starch to glucose diet orvice versa when fish were transformed from the glucose to the starch diet. Hepatic hexokinase, phosphofructokinase, and glucose-6-phosphatase activities in fish were not affected by the different dietary regimes. Malic enzyme (ME), glucose-6-phosphate dehydrogenase (G-6-PD) and phosphogluconate dehydrogenase (PGD) activities were higher in the fish fed the starch diet than in those fed the glucose diet. Changing the diet from starch to glucose decreased ME, G-6-PD and PGD activities, whereas, changing the diet from the glucose to the starch increased these enzyme activities in fish liver. These results suggest that lipogenic enzyme activity can adapt to dietary carbohydrates in the fish liver.
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PMID:Hepatic enzyme adaptation to different dietary carbohydrates in juvenile tilapiaOreochromis niloticus xO. aureus. 2419 63

The present study was aimed to evaluate the capacity of common dentex (Dentex dentex) to efficiently use dietary carbohydrates. So, the effects of different type and levels of carbohydrates on growth performance, feed utilization, fish composition, plasma metabolites and key metabolic pathways in liver and white muscle of common dentex are presented. Nine isonitrogenous (43%) and isoenergetic (22 MJ kg(-1)) diets were formulated combining three types, pregelatinized starch (PS), dextrin (Dx) and maltodextrin (Mx), and three levels (12, 18 and 24%) of carbohydrates. Growth performance was not significantly influenced by treatments. The best feed utilization was observed in 18% Mx group. Higher hepatic lipid content was found in fish fed lower dietary carbohydrate levels. PS induced higher liver and white muscle hexokinase and pyruvate kinase activities compared to the lower values observed for Mx. Malic enzyme and glucose 6-phosphate dehydrogenase in liver and white muscle were lower in Mx group. The influence of dietary carbohydrates source was more noticeable than those induced by the carbohydrate level, when glycolysis and lipogenesis pathways were considered. Common dentex is able to use properly dietary carbohydrates, although optimal dietary inclusion levels are below 24%. The greater protein-sparing effect was promoted by the less complex carbohydrate (maltodextrin) and the best feed utilization indices were obtained at intermediate levels of inclusion (18%).
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PMID:Nutritional and metabolic responses in common dentex (Dentex dentex) fed on different types and levels of carbohydrates. 2566 94