Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.6.1.44 (
AGT
)
770
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A remarkable thermal stable and oxidation-resistant mutant was obtained using the random mutagenesis PCR technique on the mutant M222A gene of
subtilisin E
. Sequencing analysis revealed an A was replaced by G at nucleotide 671 of the
subtilisin E
gene, converting the asparagine codon (AAT) to serine codon (
AGT
) at position 118. The half-life of M222A/N118S enzyme activity, when heated at 65 degrees C, was approximately 80 min while the half-life of M222A and wild-type
subtilisin E
were 13 min and 15 min, respectively. This suggested the stability of the M222A/N118S mutant was five times greater than that of the wild-type enzyme. The mutant was also as oxidation resistant as the mutant M222A of
subtilisin E
. These results indicated the M222A/N118S mutant is both an oxidation-resistant and a heat-stable variant of
subtilisin E
.
...
PMID:Thermal stable and oxidation-resistant variant of subtilisin E. 1098 70
