Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.6.1.44 (AGT)
 770 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Antibodies to Mycobacterium tuberculosis antigens H37Rv and reverse strains previously isolated from patients with sarcoidosis with granular isolates were determined in 50 patients with sarcoidosis (including 16 patients isolating granular types) and 56 patients with tuberculosis, by using ELISA and immunoblotting. Serum antibodies from patients with sarcoidosis were ascertained to more commonly react in ELISA with the antigen (ultrasound disintegrant (USDs) obtained from reverse mycobacteria isolated (initially) from patients with sarcoidosis (AGS) than with the USD of the M. tuberculosis H37Rv (AGT) and, on the contrary, serum antibodies from patients with tuberculosis more frequently reacted with the M. tuberculosis H37Rv. The spectrum of serum antibodies from patients with sarcoidosis greatly differed at immunoblotting with AGS and AGT. There was most commonly a reaction with the antigenic determinants 79, 27, 30, and 50 kDa to AGS and that of the determinants 17, 35, 32 kDa to AGT.
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PMID:[Examining of humoral immunity on mycobacteria antigens in sarcoidosis]. 950 30

The aim of this study was to detect the Mycobacterium species in the sputum samples collected from tuberculosis patients in Elazig province (located in Eastern Anatolia, Turkey), by PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method. A total of 60 samples from patients (32 male, 28 female) who were diagnosed as tuberculosis by culture positivity at Elazig Tuberculosis Control Dispensary, were included to the study. After DNA extraction and isolation from the samples, gene region encoding for 65 kDa protein of mycobacteria was amplified with specific primers (first step primers: TB1; 5'-GAG ATC GAC TGG AGG ATC C-3' and TB2; 5'-AGC TGC AGC CCA AAG GTG TT- 3', second step primers: TB1 and TB3; 5'-GTG TTG GAC TCC TCG ACG GT-3') by using seminested PCR method. According to hsp65 gene region amplification, 51 (85%) samples yielded positive results, while nine (15%) samples could not be identified. Of 51 samples, 44 (86.3%) were identified as M. tuberculosis complex, four (7.8%) were M.scrofulaceum, two (3.9%) were M. avium and one (1.9%) was M. intracellulare, in the restriction assay by Haelll of the PCR products. In order to identify the species of M. tuberculosis complex, gyrB gene region was amplified in those of 44 samples with specific primers (MTUB-f; 5'-TCG GAC GCG TAT GCG ATA TC-3' and MTUB-r; 5'-ACA TAC AGT TCG GAC TTG CG-3'), and the PCR products were restricted by Rsal and Taql enzymes. In this assay, 34 (77.3%), eight (18.2%), one (2.3%) and one (2.3%) of the 44 M. tuberculosis complex samples were detected as M. tuberculosis, M. bovis, M. microti and M. africanum, respectively. Our data indicated that at least seven different Mycobacterium species were the causative agents of tuberculosis in our region. As a result, researching for species distributions of mycobacteria in all of the parts of Turkey by molecular methods and clarifying their resistance patterns against antituberculous drugs are needed for the effective control of tuberculosis.
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PMID:[Detection of Mycobacterium species distribution in the sputum samples of tuberculosis patients by PCR-RFLP method in Elazig province]. 1768 6