Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.6.1.2 (alanine aminotransferase)
 26,722 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The purpose of the studies was to determine the activity of enzymes in the serum and pig organs with colibacteriosis in the form of oedema and stomach-intestines disease playing a diagnostic role in the determination of the organ damage degree. Studies were carried out on 29 pigs. The activity of LAP, GPT, GOT, AM, FDPA, Lp, AP and AcP was determined in the serum liver, empty intestines and kidneys of the diseased and control pigs. It resulted from the experiments that in the serum of all the diseased animals the activity of FDPA and GOT was considerably increased and that of AP decreased. So, activity determination does not allow to differentiate both forms of colibacteriosis. AM activity increases only in the serum of oedema pigs. In colibacteriosis of pigs the determination of FDPA activity is the most sensitive enzymatic test. In both diseases the activity of this enzyme increases considerable in the serum and all the studied organs. The activity of the studied enzymes shows that in both forms of collibacteriosis of pigs liver, intestines and kidneys are damaged.
Pol Arch Weter 1979
PMID:[Activity of various enzymes in the blood, liver, intestines and kidneys in spontaneous colibacteriosis of piglets]. 40 Jan 85

The effect of X-irradiation on the alanine- and aspartate aminotransferase activity in the liver, kidney and spleen of mouse. Acta Physiol. Pol. 1975, 26 (1): 95-101. The alanine- and aspartate aminotransferase (GOT and GPT) activities and the protein content were measured in the liver, kidney and spleen homogenates of mice exposed to a single whole body X-irradiation with a 900 r dose. The assays were performed in 6 h intervals during the first day and 24 h intervals from the 2nd until the 6th day after the exposure. Significant differences in the enzymatic activity were found in the course of 24 h in control animals and a marked increase of this activity was found after irradiation. This may be explained by changes in the permeability of the mitochondrial membrane for enzyme molecules.
Acta Physiol Pol
PMID:The effect of X-irradiation on the alanine--and aspartate aminotransferase activity in the liver, kidney and spleen of mouse. 113 Feb 24

1. Bicarbonate stimulates the activity of rat brain cytoplasmic and mitochondrial alanine aminotransferase (EC 2.6.1.2) probably due to the enhanced affinity for its substrates. 2. Under the same conditions, the activity of crystalline aspartate aminotransferase was inhibited. 3. The role of bicarbonate buffer in regulation of alanine aminotransferase activity and synthesis of alanine are discussed.
Acta Biochim Pol 1975
PMID:Effect of bicarbonate buffer on the activity of cytoplasmic and mitochondrial alanine aminotransferase. 117 9

Untrained rats were subjected to a single intense physical effort. In the plasma the activity of alanine aminotransferase, aspartate aminotransferase, and the concentrations of amino acids: glycine, cystine, alanine and leucine with isoleucine were measured. The results were compared with the data obtained in a control group. Despite lack of statistically significant differences in the activity of aminotransferases and concentration of amino acids between these groups a correlation was found between the activity of AIAT and alanine concentration in the animals after exercise. The concentration of alpha-amino nitrogen was decreased statistically significantly in the group of animals subjected to intensive exercise.
Acta Physiol Pol
PMID:The effect of a single intense effort on the activity of aminotransferases and concentration of free amino acids in the plasma of rats. 119 42

In 50 patients with mitral stenosis in the stage of maximal clinical improvement a correlation between the functional state of liver and the morphological changes was estblished. For this purpose the serum total bilirubin concentration was determined together with the serum total protein, albumin and globulin levels, serum alanine aminotransferase, aspartate aminotransferase, cholinesterase, leucylaminopeptidase, and alkaline phosphatase activities. The bromsulphalein test, provoked hypoprothrombinemia test, and histological examination of liver biopsy specimens were made. It was found that with increasing liver morphological lesions there is a gradual but not parallel impairment of function. The most sensitive index of hepatic functional disturbances was the test of provoked hypoprothrombinemia according to Kirchmayer and Bromowiczowa. For full assessment of the degree to which the changes in the congested liver have advanced it is necessary to perform liver function tests in combination with histological examination of the organ.
Pol Med Sci Hist Bull
PMID:Correlation of liver function and morphological abnormalities in mitral stenosis. 122 31

The activity of highly purified L-serine:glyoxylate aminotransferase (SGAT, EC 2.6.1.45) from rye seedlings was inhibited competitively by 5-aminolevulinate (ALA, Ki = 5 mM) SGAT was activated by hematin. Protoporphyrin IX and hematin inhibited irreversibly the activity of highly purified glutamate:glyoxylate aminotransferase (GGAT, EC 2.6.1.2) from rye seedlings. SGAT was found to catalyse transamination between ALA and hydroxypyruvate, whereas GGAT that between ALA and 2-oxoglutarate or pyruvate. It is suggested that SGAT is involved in the process of degradation of the excess ALA which has not been incorporated into porphyrin compounds.
Acta Biochim Pol 1992
PMID:On the possibility of involvement of glutamate:glyoxylate and serine:glyoxylate aminotransferases from rye (Secale cereale L.) seedlings in the metabolism of tetrapyrrole compounds. 129 92

The total protein and total bilirubin levels as well as the GOT, GPT, alkaline phosphatase, GGTP and beta-glucuronidase activity in the blood serum and faeces from 121 diarrhoeic, Friesian-Holstein calves, aged from 1 to 10 days were investigated. As a control the results obtained in the previous investigations were used. Simultaneously the microbiological and histopathological examinations were performed. The changes of certain parameters in the blood serum, in the first 10 days of life, were parallel in both healthy and sick animals. The increase in alkaline phosphatase, GOT and GPT activity in calves with diarrhoea was observed. These changes may suggest the liver function damage during the neonatal calf diarrhoea. The decrease in GGTP activity, in the light of relationship between this enzyme and alkaline phosphatase activity, indicate the disturbances in intestinal absorption of colostral fraction of GGTP. The activity of beta-glucuronidase may be the sensitive index of liver function and indicate the range and the intensity of necrotic and/or dystrophic lesions. The activity of GOT and GPT in the faeces may be used as an indicator of liver function in the calves with diarrhoea while the additional investigations to explain the relationship between alkaline phosphatase, GGTP and beta-glucuronidase activity in faeces and intestinal lesions are needed.
Pol Arch Weter 1991
PMID:[The biochemical profile of calves' liver in the course of diarrhea during the first 10 days of life]. 168 90

Chloroform was administered ip to Balb/c mice as a single dose ranging from 1/8 to 1 of the approximate lethal dose. At different time periods after administration, mice were sacrificed. Serum glutamate-pyruvate transaminase (SGPT) and sorbitol dehydrogenase (SDH) as well as glutathione (GSH) and malondialdehyde (MDA) levels in the liver were determined. Increased SGPT and SDH levels were found for all doses exceeding 1/8 of the approximate lethal dose. The depletion of GSH level was kept within 40% for all doses. A 2-4 fold increase of hepatic MDA level was found. The depletion of hepatic GSH and, to some extent the increase of serum SGPT and SDH, occurred in biphasic fashion. Dose-effect functions for these biochemical alterations could only be constructed for the second, delayed phase of action. It is postulated that the hepatotoxicity of chloroform is mainly dependent on radical formation in the course of biotransformation.
Pol J Occup Med Environ Health 1991
PMID:The hepatotoxic action of chloroform: short-time dynamics of biochemical alterations and dose-effect relationships. 181 48

1,4-Butanediol (BAD) was administered to male and female Wistar Imp:DAK rats by oral gavage for 28 consecutive days. Treated rats received BAD at daily doses of 5, 50 or 500 mg/kg/day. After 28 days all animals were necropsied. Blood samples were obtained and selected organs were weighed and prepared for histological examination. Subacute oral administration of BAD resulted in an overall low degree of systemic toxicity. There were no changes in body weight, food consumption, and absolute and relative organ weights. Slightly higher activities of sorbitol dehydrogenase and alanine aminotransferase were observed in male rats given BAD at the highest dose of 500 mg/kg/day. Some disturbances in hematological parameters, characterized by macrocytosis and thrombocytopenia were observed in treated rats. Mild to moderate inflammation of the liver, characterized by proliferation of bile ducts and periportal infiltrations with fibroblasts and mononuclear cells, were found in treated animals. A statistically significant difference for histopathological changes was found in animals treated with BAD at the dose of 500 mg/kg/day only in the case where both sexes were jointly taken for comparison.
Pol J Occup Med 1990
PMID:Subacute oral toxicity of 1,4-butanediol in rats. 213 25

In 100 adult patients with severe haemophilia A (78 patients) and B (22 patients) sera were screened for the presence of serological markers of hepatitis B virus (HBV) and of cytomegalovirus (CMV) and liver function tests were performed which included measurement of serum aminotransferase AST and ALT activities, total bilirubin concentration and plasma levels of factor VII and X. In all the patients at least one out of five determined HBV markers (HBsAg. HBeAg, anti-HBs, anti-HBc and anti-HBe) was detected. HBsAg was found in 10% of the patients, and its prevalence in haemophiliacs B was higher than than observed in haemophiliacs A (22.7% and 6.4%, respectively). HBsAg appeared more frequently in patients receiving factor VIII concentrates (16.7%) than in those treated with cryoprecipitate (4.5%). Anti-CMV antibody was detected in sera of 98% of the patients. In 1/3 samples of cryoprecipitate anti-HBc or anti-HBs were present, and in the half of samples anti-CMV occurred. Abnormal liver function tests indicating chronic hepatitis or liver cirrhosis were obtained in 8 patients. Raised ALT activity which could suggest chronic infection with non-A, non-B virus occurred in 6 cases. The present study indicates that haemophiliacs frequently transfused with plasma products are at high risk for viral infections leading to liver dysfunction.
Acta Haematol Pol
PMID:[Serological markers of hepatitis B virus and cytomegalovirus in patients with hemophilia]. 217 33


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