Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.6.1.2 (alanine aminotransferase)
 26,722 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Changes in serum chemistry values as a result of incomplete removal of erythrocytes and in vitro hemolysis during the preparative process have been studied. Two levels of contamination, corresponding to removal of 99% and 99.9% of the erythrocytes, were used to examine the effects of both hemolyzed and intact cells. Forty chemical procedures and methods were considered. Serum LDH values were most strongly affected by hemolyzed erythrocytes. Potassium, creatine phosphokinase, aspartate aminotransferase, alanine aminotransferase, and iron showed smaller but significant effects due to the presence of 1% hemolyzed cells, with lesser effects observed at the 0.1% level. The presence of non-hemolyzed cells at either level did not significantly alter chemistry results.
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PMID:The effects of 0.1 and 1.0 per cent erythrocytes and hemolysis on serum chemistry values. 97 Mar 64

In our studies on patients with head injury, it was noted that there are some correlations between their clinical courses and the urinary excretion of creatine (cr), creatinine (Crn), 17-ketosteroid and 17-hydroxycorticosteroid. We observed the high urinary excretion of Cr in patients with severe head injury while almost negative in a mild case. We reported those facts in 1974. Also noted in patients with head injury is the relationship between the enzyme-activities (GOT, GPT, LDH and CPK) in the cerebrospinal fluid and their clinical courses. In this paper, we reported 34 cases of head injured patients (simple type: 2, concussion: 9, contusion: 8, acute intracranial hematoma: 7 and chronic intra-cranial hematoma: 8). The control values of CSF enzyme-activities were determined in these 14 cases (simple head injury, whip-lash injury and osteoma of the skull) as GOT less that 15, GPT less than 7, LDH less than 12 and CPK less than 8 units. In the moderate cases, a slight increase in activities of 4 enzymes in CSF were observed, while in severe or comatose cases, the enzyme-activities (especially LDH and CPK) were greater than in the controls. In the dead cases these values were five times as high as the normal case. In the patients recovering from a serious stage, these activities decreased to normal. High CSF enzyme-levels tend to indicate a poor prognosis and low levels a favorable progrosis. In the patients with a significant elevation of CSF enzymes, a high urinary excretion of Cr [normal range: 0-150 (ca. 50)mg/day] was often observed. There was no apparent correlation between the enzyme level in CSF and that in serum and the increase or decrease of these 4 enzymes are not always proprotionate with each other. As reported by Green (1958) and Lending (1961), cerebral cell necrosis and increased permeability of BLB, BBB or cerebral cell membrane can be related to the increase of enzymeactivities. With these observations, it can be considered that severe head injury gives influence on metabolic function in the hypothalamus and may cause in the levels of CSF enzymes and/or the urinary excretions of Cr, Crn and corticosteroids. And the examinations of enzyme activities in the patients with head injury may become a useful aid to make an outlook of their clinical coure and prognosis.
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PMID:[CSF enzyme activities in patients with head injury--especially on GOT, GPT, LDH, and CPK (AUTHOR'S TRANSL)]. 98 69

A description is given of an outbreak of equine infectious anaemia (E.I.A.) in Campania [at Naples and Aversa (Caserta)]; it was diagnosed by clinical, pathological and serological examinations (Coggins test). Using the serum of 45 horses with E.I.A. and 11 healthy horses (controls), numerous investigations were carried out on: enzymes, intrinsic coagulation factors, lipids and other substances. The results obtained were very interesting and show that in this disease there are significant increases in many enzymes (LDH, LAP, gamma-GT, CPK, PK and ALD) and copper. Insignificant increases were found in other enzymes (SDH, GLDH, MDH, ICDH, AIP, lysozyme, cholinesterase, GOT and GPT) and also intrinsic coagulation factors, lipid substances (total cholesterol, esterified cholesterol, triglycerides) and glucose. LDH-1-isoenzyme remains unchanged, whilst AcP decreases slightly.
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PMID:Biochemical studies on equine infectious anaemia. 101 May 2

The study was carried out on rats of the Wistar strain with experiments hypertension by the method of Selye, modified by Kolarova. These authors examined the activity of the enzymes of protein metabolism (GOT, GPT, AP), of glycolysis (ALD, LDH, alpha-HBDH) and of the citric cycle (MDH) in the left and right kidney at various intervals after modeling the process (3th, 15th, 30th and 90th day). The activity of the enzymes of the glycolytic chain and the cycle of Krebs in the left kidney was lowered at all stages of the experiment. Transaminase activity diminished progressively. Such a dynamics revealed the activity of adenosine phosphate as well. The activity of all examined enzymes of the right kidney showed phase changes-after initial elevation on the third day there was a lowering at the later stages of the experiment. The altered activity off the left kidney was connected with the disturbed vascularization with subsequent metabolic changes. The changes in the enzymic activity in the right kidney were assumed as a consequence of the occurred adaptive hyperfunction.
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PMID:[Changes in renal enzyme activity in rats with experimental hypertension]. 102 9

Serum activity analyses of nine enzymes [CPK, HBDH, SDH, ALP, GPT, GOT, LDH, MDH, ALD] in patients with rheumaitc carditis revealed certain elevations of the mean values of LDH, GOT, HBDH, GPT, ALP and MDH. These changes have no particular diagnostic importance because similar findings were also obtained in patients with both rheumatic and nonrheumatic arthritis. The changes occurring during antirheumatic treatment are minimal and statistically not significant. Greater elevations of LDH, ALD, GOT, HBDH, GPT, MDH, and ALP are found in patients with decompensated rheumatic heart disease associated with active rheumatic carditis.
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PMID:Changes in blood-serum enzymes in rheumatic myocarditis. 102 12

Gastric and duodenal ulcers were characterized by the high activity of alkaline phosphatase within the zone of impairment. In gastric ulcer the high concentration of the enzyme was observed also in the region of minor curvature. Activities of aspartate and alanine transaminases were decreased in the zone of gastric ulcer; in duodenal ulcer only the alanine transaminase activity was decreased. The total lactate dehydrogenase activity was unaltered in gastric ulcer, but it was increased in impaired duodenum, where, among the other LDH isozymes, LDH5 fraction was increased.
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PMID:[Non-proteolytic enzymes of human gastric and duodenal mucosa in peptic ulcer]. 103 Aug 92

The study on the embryotrophic action of phosalon insecticide preparation is conducted against the background of its comprehensive and thorough hygiene-toxicologic assay. Pregnant white rats undergo treatment per os with 1/10 and 1/100 LD50 throughout the entire gravidity period. Recordings are made of the quantity of corpora lutea, pre- and postimplantation lethality; the pedigree development is traced up to the end of the second postnatal month with readings being made of weight and length in the first postnatal day, presence or not of external malformations, weight increase and survival up to the end of the lactation period, day of eye cleft opening and hairing, and sex ratio. The presence of phosalon and phosalon-oxone in whole embryo homogenates is estimated. Enzyme activity of GOT, GPT, catalase, LDH, G-6-PD, RNA and DNA quantity are studied in the liver of newborns on the second postnatal day. Within 21 days of birth, the peripheral blood picture and visceral weight coefficient are also studied. Investigation of the peripheral blood picture, activity of catalase, GOT and GPT in the liver and serum, alkaline and acid phosphatase, and peroxidase in the serum is performed at the end of the second month. The results point to the presence of an embryotoxic effect at the level of the investigated hepatic indicators, detected on the 2nd day of the postnatal period, only at 1/10 LD50 dose.
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PMID:[Embryotropic action of phosalone]. 103 14

The influence of Uden, exerted on the activity of GOT, GPT, LDH acid phosphatase and alkaline phosphatase in the serum, is investigated upon 4-day-long treatment. The experiment is conducted in three groups of white male rats with mean weight 270 +/- 20 g. The first group of animals underwnt poisoning with 1/20 LD50, the second--with 1/4 LD50 while in the third, control group, the animals were treated with an oil solvent at the respective days. It is found that acid phosphatase, GOT and alkaline phosphatase activity in group two is virtually unchanged, whilst GPT activity in both experimental groups, and acid phosphatase in group one are reliably increased (P less than 0,01), with 20,17 and 53 per cent respectively. LDH activity is reliably lowered with 60 per cent in group two, whereas in group one a tendency for a reduction is noted (P less than 0,05).
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PMID:[Changes in enzymatic activity in rats with acute Unden poisoning]. 103 21

Blood samples from rats, rabbits and beagles were subjected to simultaneous analyses for ten parameters using the RaBA-System and the results were compared with those obtained by the routine manual methods. Although the mean values measured by the two methods differ in several items, a significant correlation between the two methods in the majority of the determinations makes the use of the RaBA-System acceptable. The RaBA-System is thus considered to be applicable to evaluation of hemoglobin, total protein, glucose, BUN, total cholesterol, GOT, GPT, and alkaline phosphatase in blood samples from rats, rabbits and beagles. Plasma LDH and albumin, however, cannot be determined with accuracy by the system.
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PMID:[Application of the RaBA-System to clinical blood chemistry test in experimental animals]. 103 65

Serum GOT, GPT, LDH, CHE, MDH, SDH, PK, GLDM, LAP and amylase levels and serum ammonia were determined in 49 major burn cases with 2nd and 3rd degree lesions covering 25% to 80% of the body surface. GOT, GPT, LDH, CHE and gamma-GT have above normal in all cases. Limited and inconsistent movements were noted for the remaining enzymes. Blood ammonia was constantly increased and it is felt that the enzyme picture may be of considerable prognostic value in major burn cases.
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PMID:[Serum enzyme picture in major burns]. 111 73


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