Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.6.1.2 (alanine aminotransferase)
 26,722 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. Factors regulating the release of alanine and glutamine in vivo were investigated in starved rats by removing the liver from the circulation and monitoring blood metabolite changes for 30 min. 2. Alanine and glutamine were the predominant amino acids released into the circulation in this preparation. 3. Dichloroacetate, an activator of pyruvate dehydrogenase, inhibited net alanine release: it also interfered with the metabolism of the branched-chain amino acids valine, leucine and isoleucine. 4. L-Cycloserine, an inhibitor of alanine aminotransferase, decreased alanine accumulation by 80% after functional hepatectomy, whereas methionine sulphoximine, an inhibitor of glutamine synthetase, decreased glutamine accumulation by the same amount. 5. It was concluded that: (a) the alanine aminotransferase and the glutamine synthetase pathways respectively were responsible for 80% of the alanine and glutamine released into the circulation by the extrasplanchnic tissues, and extrahepatic proteolysis could account for a maximum of 20%; (b) alanine formation by the peripheral tissues was dependent on availability of pyruvate and not of glutamate; (c) glutamate availability could influence glutamine formation subject, possibly, to renal control.
Biochem J 1975 Sep
PMID:Factors regulating amino acid release from extrasplanchnic tissues in the rat. Interactions of alanine and glutamine. 0 55

Both gamma-glutamyltransferase (gammaGT) and pseudocholinesterase (PCE) were found to be increased in hypertriglyceridemic subjects. High values of gammaGT were noted in alcoholic subjects and especially in those with either increased serum triglyceride or alanine aminotransferase higher than 16 mU/ml, while PCE was not significantly changed in alcoholic subjects. Although both enzymes were strongly correlated with the logarithm of serum triglyceride and the prebeta electrophoretic fraction, there were striking differences concerning their behavior in various hypertriglyceridemic subjects. PCE activity was high even in moderate hypertriglyceridemias but its correlation with serum triglyceride had a tendency to flatten with increasing concentration of triglyceride. However, increase of gammaGT was rather characteristic for gross hypetriglyceridemia. Short-term, triglyceride-lowering therapy was accompanied by a tendency to normalization of gammaGT, while PCE values were not influenced. An attempt was made to interpret these changes of serum-enzyme activity in hypertriglyceridemia in connection with mechanisms of lipoprotein synthesis and with the pathogeny of hyperlipemic conditions.
Clin Chim Acta 1976 Sep 20
PMID:Increased serum gamma-glutamyltransferase in hypertriglyceridemia: comparison with serum pseudocholinesterase. 0 18

One hundred and seven Wistar rats, 8 weeks old and weighing 180-200 g, were housed under conditions of controlled temperature (22 plus or minus 2 degrees) and lighting (light on from 07:00 to 19:00). They were divided into 2 groups and fed diets containing either 15 per cent cas-protein for 23 days. Food consumption was recorded every 2 hours for each animal during 48 hours. Four or five rats from each group were killed every 2 hours for 24 hours and the hepatic activities of PK (EC.2.7.1.40),G6P-DH (EC1.1.1.49), ME (EC1.1.1.40), Acetyl-CoA-carbox (EC.6.4.1.2.),PC(EC.6.4.1.1.), PEP-CK(EC.4.1.1.32), G6Pase (EC.3.1.3.9) and GPT (EC.2.6.1.2.) were measured...
J Physiol (Paris) 1976 Sep
PMID:[Dietary protein level and circadian variation of enzyme activities for glucose metabolism and lipogenesis in male rats (author's transl)]. 1 10

Details of a systematic approach to suitability testing of commercial control sera are given for substrate optimized L-aspartate aminotransferase and L-alanine aminotransferase methods at 37 degrees C. Their acceptability for control purposes of standardized methods depends on: (1) the range of control values in relation to borderline values, (2) stability, (3) aspect, clarity, (4) NADH consumption in preincubation time, (5) blank activities, (6) kinetic data as half saturation constants and saturation curves, (7) influence of effectors, (8) isoenzyme pattern. These evaluation criteria are proposed for suitability testing. The term "representativeness" should be introduced as a special criterion for main characteristics of control materials. The authors want to point out the close connection with standardization of methods.
Clin Chim Acta 1977 Sep 15
PMID:Suitability of commercial enzyme control sera for the quality control of activity determinations of L-aspartate aminotransferase and L-alanine aminotransferase in human serum. 1 83

The authors present the results of observations over 407 children aged from 2 months to 16 years from the foci of viral hepatitis in children's collective bodies. During the quarantine a determination was made in children of the glutamic-pyroracemic, glutamic-oxalic transaminases (GPT and GOT, respectively) and of the hepatitis B antigen (HBAg). A necessity of using the enzymatic tests for the purpose of early diagnosis of viral hepatitis was shown, since 84% of the cases developing in the next focus coursed as an unicteric form without any markked clinical signs; HBAg was revealed in 6.1% of the children examined. A complex examination of the personnel and of the persons who came in contact with the patients with viral hepatitis showed the ways of spread of hepatitis B in a collective body; it was found that the viral hepatitis B infection took place both by parenteral and enteral routes. The expediency of active observation over the children, recipients of blood and plasma, with determination in them of the activity of the enzymes and HBAg for early diagnosis of parenteral infection was substantiated. It was also shown that the incidence of the unicteric forms of viral hepatitis in a focus of infection depended not on the periods of gamma-globulin administration but on the age of children who contracted the infection. Thus, the prevalence of the unicteric forms of the disease over the icteric ones in children under 3 years of age was more pronounced than in older children.
Zh Mikrobiol Epidemiol Immunobiol 1975 Sep
PMID:[Spread of viral hepatitis in organized children's collectives and the methods for its early diagnosis]. 5 73

In 110 children-between 0-16 years of age-, 90 children with Down-syndrome and 20 controls the following metabolic parameter were analyzed: ETK (vitamin-B1-activating coefficient), EGR (vitamin B2), P-5'-P, EGOT (vitamin B6), GOT, GPT, pH, K, Na, Ca, Cl, uric-acid (HS). Among some important correlations between the different parameters it could be demonstrated-for the first time to our knowledge-that in Mongoloids a disturbance of the vitamin-B1-metabolism exists, certified by the so-called transketolase-test.
Fortschr Med 1975 Sep 11
PMID:[Studies on the state of vitamins B1, B2 and B6 in Down's syndrome]. 12 24

Twelve marmosets (Saguinus mystax) were inoculated intravenously (iv) with hepatitis A virus (HAV). One died early (day 12); seven were sacrificed at the time of elevation in level of alanine aminotransferase (serum glutamic-pyruvic transaminase), and four without elevation were not sacrificed but seroconverted. In the seven marmosets sacrificed during the acute stage of illness, hepatitis A antigen (HA Ag) was detected in the liver by immunofluorescence in all cases, by immune electron microscopy in four, and by enzyme-linked immunosorbent assay (ELISA) in three. The HA Ag appeared by immunofluorescence as very fine granules in the cytoplasm of hepatocytes and Kupffer cells. The HA Ag could not be detected by immunofluorescence in biopsy specimens taken from the duodenum, jejunum, ileum, or transverse colon in any of eight marmosets in which necropsy was performed during the acute or preacute stage of illness. These findings suggest that the gut is not involved during the acute phase of HAV infection following iv inoculation into marmosets. The ELISA results showed that only three of 12 marmoset livers obtained during the acute phase of HAV infection could be used as an antigen source in serologic testing for antibody to HA Ag. Thus, marmoset livers were no better as a source of HA Ag than acute-phase stools from patients with type A hepatitis.
J Infect Dis 1978 Sep
PMID:Localization of hepatitis A antigen in marmoset organs during acute infection with hepatitis A virus. 21 88

The quantitative differences between the activity of the 3 common phenotypes of human red cell GPT has been confirmed. In addition, the activity of red cell GPT 1 was found to be greater in young children than in adults. No such difference was found for the GPT 2 phenotype. The activity of the red cell GPT 1 was found to decrease with age, reaching the adult level at the age of 10 to 12 years. Red cell GPT of all the 3 common phenotypes in both adults and children was found to show a similar response to the addition of excess pyridoxal phosphate. A method has been devised for the partial purification of human GPT (cytoplasmic) from liver. GPT 1 and GPT 2 have been purified, and very few significant differences were found amongst the physical and kinetic parameters tested.
Humangenetik 1975 Sep 20
PMID:Comparative studies on the human glutamate-pyruvate transaminase phenotypes--GPT 1, GPT 2-1, GPT 2. 24 1

In five children affected by HBsAg positive chronic persistent hepatitis a treatment with Levamisole (LMS) modified several immunological parameters which had been found altered before treatment. In particular the percentage of E rosette forming cells increased while that of EAC decreased; B lymphocytes with SmIgG and the responsiveness to phytohaemagglutinin, which were significantly decreased and increased respectively, got normal values. Since the persistence of HBsAg was unaffected by LMS treatment and a light increase of transaminase serum levels (GPT and GOT) was observed during treatment, doubts are expressed about the opportunity of using LMS in children affected by such a form of hepatitis.
Boll Ist Sieroter Milan 1978 Sep 30
PMID:[Chronic persistent HBsAg positive hepatitis in children. II. Effect of treatment with levamisole]. 31 Jun 86

A rabbit model was used to determine the effects of prostaglandins and arachidonic acid on cellular integrity and survival during endotoxic shock. Prostaglandins A2, E1 and F2alpha were infused intravenously at a rate of 1.0 microgram/kg/min for 105 min beginning 15 min after the administration of an LD60 dose of Escherichia coli endotoxin. While each of the prostaglandins tested significantly attenuated the accumulation of lactic acid dehydrogenase in the plasma of shocked animals, none were able to protect against the increase in the plasma activities of glutamic pyruvic transaminase or cathepsin D during the shock state. Prostaglandins A2, E1 and F2alpha did not significantly enhance the survival of the treated animals as compared to vehicle-treated controls. In contrast, arachidonic acid 15 microgram/kg/min i.v.) significantly prevented the accumulation of lactic acid dehydrogenase and glutamic-pyruvic transaminase activities in the plasma of shocked animals, and also significantly increased the number of survivors in this group 48 hours after the endotoxin administration. In summary, while the treatment of endotoxic rabbits with prostaglandins of the A, E and F series was of no survival value, the treatment of these animals with a substrate of the prostaglandin synthetase complex resulted in a dramatic increase in the survival rate. The mechanism of action of arachidonic acid in this regard is not clear.
J Pharmacol Exp Ther 1978 Sep
PMID:Endotoxic shock in the rabbit: the effects of prostaglandin and arachidonic acid administration. 35 77


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