Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.6.1.19 (
GABA transaminase
)
808
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The effect of dietary protein on pyrimidine-metabolizing enzymes was studied in the rat. The activities of dihydropyrimidine dehydrogenase and
beta-ureidopropionase
in the livers of rats fed a protein-free diet were significantly decreased, while the activity of dihydropyrimidinase was unaffected. Protein deficiency (5%) also decreased the activity of
beta-ureidopropionase
. On the other hand, a high-protein diet (60%) increased the level of
beta-ureidopropionase
. The activities of
beta-alanine-oxoglutarate aminotransferase
(
aminobutyrate aminotransferase
) and D-3-aminoisobutyrate-pyruvate aminotransferase ((R)-3-amino-2-methylpropionate-pyruvate aminotransferase), which are present in mitochondria, depended on the amount of protein in the diet. Ammonium ions supplemented in the diet and given by injection did not affect the activities of rat liver pyrimidine-metabolizing enzymes (dihydropyrimidine dehydrogenase, dihydropyrimidinase,
beta-ureidopropionase
,
beta-alanine-oxoglutarate aminotransferase
and D-3-aminoisobutyrate-pyruvate aminotransferase). Dietary uridine resulted in the accumulation of uracil in the liver, but did not affect the activities of pyrimidine-metabolizing enzymes.
...
PMID:Effect of dietary protein on pyrimidine-metabolizing enzymes in rats. 180 76
The effect of 6-azauracil on beta-alanine metabolism was investigated in vivo in the rat. Both of the enzymes
beta-alanine-oxoglutarate aminotransferase
(
aminobutyrate aminotransferase
) and D-3-aminoisobutyrate-pyruvate aminotransferase [R)-3-amino-2-methylpropionate-pyruvate aminotransferase), which are beta-alanine catabolizing enzymes from rat liver and kidney, were inactivated by 6-azauracil injection, while dihydrouracil dehydrogenase, dihydropyrimidinase, and
beta-ureidopropionase
, which are pyrimidine metabolizing enzymes, were not affected. The content of beta-alanine was increased, but the level of uridine and uracil in rat liver was not affected, by 6-azauracil. When a crude enzyme preparation was passed through a Sephacryl S-200 column, both enzymes could be separated from each other. beta-Alanine-oxoglutarate aminotransferase and beta-alanine-pyruvate aminotransferase activities in rat liver decreased to 27.4% and 63.9%, respectively, upon 6-azauracil injection, and those in kidney were 11.7% and 38.3%, respectively. From these findings, it is suggested that the accumulation of beta-alanine in 6-azauracil-treated rat liver might be caused by the inhibition of beta-alanine catabolizing enzymes, but not by an increase in the uridine pool nor by the activation of pyrimidine metabolism.
...
PMID:Inhibitory effect of 6-azauracil on beta-alanine metabolism in rat. 263 79
beta-Alanine-oxoglutarate aminotransferase from rat liver was mainly distributed in the mitochondrial fraction, while
beta-ureidopropionase
, the last one of uracil-metabolizing enzymes to beta-alanine, was predominantly distributed in the cytosolic fraction. When rat liver mitochondria were separated into submitochondrial fractions,
beta-alanine-oxoglutarate aminotransferase
was localized in the mitochondrial matrix. beta-Alanine was transported into mitochondria with time and the influx of beta-alanine into the matrix was 0.47 nmol/mg of mitochondrial protein at 2 min after incubation.
...
PMID:Submitochondrial localization of rat liver beta-alanine-oxoglutarate aminotransferase. 313 42
