Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.6.1.1 (aspartate aminotransferase)
 21,665 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A five-step procedure is described for preparing highly purified aspartate aminotransferase (L-aspartate: 2-oxoglutarate aminotransferase, EC.2.6.1.1) from cell-freee enzyme extracts of Pediococcus cerevisiae. An overall purification of 130-fold was achieved. Some of P. cerevisiae aspartate aminotransferase properties were studied, i.s. pH optimum (7.8--8.0), optimum of temperature (37 degrees), Michaelis constans for 4 enzyme substrates and substrate specificity of enzyme. The enzyme is very thermolabile. During purification the enzyme was stabilizated by 2-oxoglutarate. The highly purified preparation was stored in the solution containing ammonium sulphate. The obtained aspartate aminotransferase preparation was free of alanine and aromatic amino acids aminotransferase activites and did not reveal malate dehydrogenase activity.
Acta Microbiol Pol 1977
PMID:Aspartate aminotransferase of Pediococcus cerevisiae. 6 56

Liver glycogen content and activity of several hepatic enzymes in repeatedly immobilized rats. Acta Physiol. Pol., 1978, 29 (1): 55-60. In adult, male rats subjected to immobilization for 2.5 h daily, on the 7th day, 24 h after a sixth trial, an increased activity of the liver aspartate aminotransferase was found as shown by comparing their data with those of normally fed controls and also of the animals pair fed to the immobilized. Moreover, a net increasing effect of daily immobilization on the liver glycogen content was described.
Acta Physiol Pol
PMID:Liver glycogen content and activity of several hepatic enzymes in repeatedly immobilized rats. 66 49

The effect of X-irradiation on the alanine- and aspartate aminotransferase activity in the liver, kidney and spleen of mouse. Acta Physiol. Pol. 1975, 26 (1): 95-101. The alanine- and aspartate aminotransferase (GOT and GPT) activities and the protein content were measured in the liver, kidney and spleen homogenates of mice exposed to a single whole body X-irradiation with a 900 r dose. The assays were performed in 6 h intervals during the first day and 24 h intervals from the 2nd until the 6th day after the exposure. Significant differences in the enzymatic activity were found in the course of 24 h in control animals and a marked increase of this activity was found after irradiation. This may be explained by changes in the permeability of the mitochondrial membrane for enzyme molecules.
Acta Physiol Pol
PMID:The effect of X-irradiation on the alanine--and aspartate aminotransferase activity in the liver, kidney and spleen of mouse. 113 Feb 24

1. Bicarbonate stimulates the activity of rat brain cytoplasmic and mitochondrial alanine aminotransferase (EC 2.6.1.2) probably due to the enhanced affinity for its substrates. 2. Under the same conditions, the activity of crystalline aspartate aminotransferase was inhibited. 3. The role of bicarbonate buffer in regulation of alanine aminotransferase activity and synthesis of alanine are discussed.
Acta Biochim Pol 1975
PMID:Effect of bicarbonate buffer on the activity of cytoplasmic and mitochondrial alanine aminotransferase. 117 9

Untrained rats were subjected to a single intense physical effort. In the plasma the activity of alanine aminotransferase, aspartate aminotransferase, and the concentrations of amino acids: glycine, cystine, alanine and leucine with isoleucine were measured. The results were compared with the data obtained in a control group. Despite lack of statistically significant differences in the activity of aminotransferases and concentration of amino acids between these groups a correlation was found between the activity of AIAT and alanine concentration in the animals after exercise. The concentration of alpha-amino nitrogen was decreased statistically significantly in the group of animals subjected to intensive exercise.
Acta Physiol Pol
PMID:The effect of a single intense effort on the activity of aminotransferases and concentration of free amino acids in the plasma of rats. 119 42

In 50 patients with mitral stenosis in the stage of maximal clinical improvement a correlation between the functional state of liver and the morphological changes was estblished. For this purpose the serum total bilirubin concentration was determined together with the serum total protein, albumin and globulin levels, serum alanine aminotransferase, aspartate aminotransferase, cholinesterase, leucylaminopeptidase, and alkaline phosphatase activities. The bromsulphalein test, provoked hypoprothrombinemia test, and histological examination of liver biopsy specimens were made. It was found that with increasing liver morphological lesions there is a gradual but not parallel impairment of function. The most sensitive index of hepatic functional disturbances was the test of provoked hypoprothrombinemia according to Kirchmayer and Bromowiczowa. For full assessment of the degree to which the changes in the congested liver have advanced it is necessary to perform liver function tests in combination with histological examination of the organ.
Pol Med Sci Hist Bull
PMID:Correlation of liver function and morphological abnormalities in mitral stenosis. 122 31

In 93 alcohol dependent patients following laboratory tests were done: gamma-glutamic transpeptidase (GGTP), aspartate aminotransferase (SGOT), mean erythrocyte volume (MCV), triglycerides (TGL), and HDL-cholesterol to whole-cholesterol ratio (HDLC%). The psychometric evaluation was made by MAST questionnaire and by authors clinical scale for the evaluation of alcohol dependence. Lover values of GGTP and SGOT were shown in patients during abstinence than in subjects continuing drinking. Patients more severely dependent showed higher HDLC%. The more frequent abnormalities reflected: GGTP (33.7%), MCV (33.7%), and HDLC (31.4%). The use of these 3 markers allowed to reveal abnormalities in 72.1% of subjects, while the use of all 5 markers - in 81.4%. The identification significance of markers was different in persons with more and less severe alcohol dependence. Three most sensible markers in the group of less dependent subjects were GGTP, MCV, TGL (60% of subjects showed abnormalities regarding these markers). Among more severe dependent subjects HDLC%, GGTP, and MCV were most sensible markers (78.4% of abnormalities). Using all 5 markers the abnormalities in the group of less severe dependent subjects were found in 71.4%, and in the group of more severe dependent patients--in 88.2%.
Psychiatr Pol
PMID:[Biochemical markers in relation to the degree of alcohol dependence]. 198 82

Activities of alanine and aspartate aminotransferase in different muscle types and in the liver of streptozotocin diabetic rats were studied 1,2 and 3 days after administering of streptozotocin. It was shown that the activity of both enzymes was elevated in the "white" layer of the vastus lateralis, in the liver and in the heart, whereas it remained unchanged in the "red" layer of the same muscle, in the soleus and the diaphragm. It is concluded that the effect of acute insulin deficiency on the aminotransferase activity in skeletal muscles depends on the muscle fiber composition and does not appear in muscles with a high oxidative potential. These results indicate that muscle fiber composition should be taken into account when evaluating the role of insulin in amino acid metabolism in the muscle.
Acta Physiol Pol 1990
PMID:Alanine and aspartate aminotransferase activities in muscles of diabetic rats. 213 88

In 100 adult patients with severe haemophilia A (78 patients) and B (22 patients) sera were screened for the presence of serological markers of hepatitis B virus (HBV) and of cytomegalovirus (CMV) and liver function tests were performed which included measurement of serum aminotransferase AST and ALT activities, total bilirubin concentration and plasma levels of factor VII and X. In all the patients at least one out of five determined HBV markers (HBsAg. HBeAg, anti-HBs, anti-HBc and anti-HBe) was detected. HBsAg was found in 10% of the patients, and its prevalence in haemophiliacs B was higher than than observed in haemophiliacs A (22.7% and 6.4%, respectively). HBsAg appeared more frequently in patients receiving factor VIII concentrates (16.7%) than in those treated with cryoprecipitate (4.5%). Anti-CMV antibody was detected in sera of 98% of the patients. In 1/3 samples of cryoprecipitate anti-HBc or anti-HBs were present, and in the half of samples anti-CMV occurred. Abnormal liver function tests indicating chronic hepatitis or liver cirrhosis were obtained in 8 patients. Raised ALT activity which could suggest chronic infection with non-A, non-B virus occurred in 6 cases. The present study indicates that haemophiliacs frequently transfused with plasma products are at high risk for viral infections leading to liver dysfunction.
Acta Haematol Pol
PMID:[Serological markers of hepatitis B virus and cytomegalovirus in patients with hemophilia]. 217 33

In 34 patients (16 women and 18 men) with acute leukaemias (8 with acute lymphoblastic leukaemia and 26 with acute myeloblastic leukaemia), as yet untreated, the serum levels were determined of conjugated cholic acid, bilirubin, aspartate aminotransferase (AspAT), alanine aminotransferase (AlAT), alkaline phosphatase (AP), lactate dehydrogenase (LDH) and cholinesterase (Chol). Serum conjugated cholic acid level was determined by radioimmunoassay. The mean values of AP and Chol activity were within the range of normal values in this laboratory, the values of AspAT and AlAT were slightly above this range, and LDH value exceeded twice this normal range. The mean bilirubin concentration was within normal range. The greatest changes were noted in conjugated cholic acid values, the mean value exceeded five times the upper normal range (1.0 mumol/l). In 30 patients (88%) the conjugated cholic acid level in the serum was above 1.0 mumol/l, in the remaining 4 cases it was above the mean value for the control group. No correlation was found between conjugated cholic acid and any of the determined parameters. These results point out that the serum level of conjugated cholic acid may be a valuable parameter for assessment of hepatocellular function in acute leukaemias.
Pol Arch Med Wewn
PMID:[Serum cholic acid levels in patients with acute leukemia]. 225 Dec 7


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