Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.6.1.1 (aspartate aminotransferase)
 21,665 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Based on a feeding trial using 27 lactating "Simmental-cows" the effect of naturally contaminated feed with deoxynivalenol (DON) as well as zearalenone (ZON) regarding production parameters was examined. 3 groups of cows according to lactation number, milk yield (kg ECM) and body mass were used. The average daily intake of DON in group K was 12.4 mg, in group T 14.1 mg and in group M 14.3 mg and ZON in group K was 12.4 mg, in group T 0.67 mg and in group M 0.68 mg respectively. The feed of animals of group M was supplemented with "Mycofix Plus" as mycotoxin inactivator. The red and white blood picture including the thrombocytes were in all groups within the normal range. Concerning enzymes (GGT, AP) and metabolites (GLUC, TBIL, UREA, CREA) the mean values of the 3 groups were in the normal range. Slightly increased were the mean values of all groups in respect to the AST- and GLDH-activities. Volatile fatty acids of the rumen content were significantly highest in group M, also the number of dead rumen infusoria was significantly decreased, but the counts of small sized infusoria increased. The study has shown that "Mycofix Plus" might be able to enhance the activity of rumen flora concerning detoxification of mycotoxins in feed of dairy cows.
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PMID:[Effect of mycotoxin contaminated feed on production parameters of dairy cows]. 1068 79

MRP3 is an ABC transporter localized in the basolateral membrane of epithelial cells such as hepatocytes and enterocytes. In this study, the role of Mrp3 in drug disposition was investigated. Because Mrp3 preferentially transports glucuronide conjugates, we investigated the in vivo disposition of acetaminophen (APAP) and its metabolites. Mrp3+/+ and Mrp3-/- knockout mice received APAP (150 mg/kg), and bile was collected. Basolateral and canalicular excretion of APAP was also assessed in the isolated perfused liver. In separate studies, mice received 400 mg APAP/kg for assessment of hepatotoxicity. No differences were found in the biliary excretion of APAP, APAP-sulfate, and APAP-glutathione between Mrp3+/+ and Mrp3-/- mice. However, 20-fold higher accumulation of APAP-glucuronide (APAP-GLUC) was found in the liver of Mrp3-/- mice. Concomitantly, plasma APAP-GLUC content in Mrp3-/- mice was less than 10% of that in Mrp3+/+ mice. In addition, APAP-GLUC excretion in bile of Mrp3-/- mice was tenfold higher than in Mrp3+/+ mice. In the isolated perfused liver, we also found a strong decrease of APAP-GLUC secretion into the perfusate of Mrp3-/- livers. Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), and histopathology showed that Mrp3-/- mice are more resistant to APAP hepatotoxicity than Mrp3+/+ mice, which is most likely a result of the faster repletion of hepatic GSH. In conclusion, basolateral excretion of APAP-GLUC in mice is nearly completely dependent on the function of Mrp3. In its absence, sufficient hepatic accumulation occurs to redirect some of the APAP-GLUC to bile. This altered disposition in Mrp3-/- mice is associated with reduced hepatotoxicity.
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PMID:Altered disposition of acetaminophen in mice with a disruption of the Mrp3 gene. 1625 50