Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.6.1.1 (aspartate aminotransferase)
 21,665 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Sixty-seven clinically healthy mature coypus were studied for some biochemical values in blood, 25 coypus were examined for the distribution of serum proteins and 20 ones for haematological values. Blood was sampled when the animals were killed and was examined by current laboratory methods. In comparison with the other animals, coypus had higher values of phosphorus and magnesium, and lower values of total protein and gamma globulins. The activity of enzymes (AST, ALT, GMT, ALP) was at the same level as in the other animals. Lower values of haemoglobin were found in coypus; the remaining haematological values are close to those in the other animals.
 ...
PMID:[Biochemical and hematological values in the blood of the nutria]. 641 18

Single biochemical analyses can be used for the diagnosis of animal diseases only with the knowledge of the effects that may distort the single result. The study of the repeatability of analyses is described in the four basic enzymes (AST, ALP, GMT, LD), which are most frequently used for diagnosis. The experiment was conducted in a group of ten Kladrub mares. Six blood samples were taken from each of the mares within ten days. The measured values were subjected to statistical processing and repeatability coefficients (r op) were calculated. All the r op values were high (ALP--0.96, LD--0.93, GMT--0.90, AST--0.60). The obtained repeatability coefficients are discussed for each enzyme, including their sources and diagnostic function.
 ...
PMID:[An analysis of reproducibility in the determination of the activity of selected enzymes in the blood serum of horses]. 642 28

Twenty-six parameters of clinical biochemical properties were determined in 72 clinically healthy German Shepherd dogs. The standard values were determined for total protein, protein spectrum, albuminoglobulin quotient, enzymic activities for AST, ALT, LD, LD-1, and ALP, for sodium, potassium, magnesium, calcium, inorganic phosphorus, and chlorides, complete prameters of the acid-base balance of the blood, and the values of glucose, urea, lactic acid, and creatinin. For determining these standards, dogs were selected at the age of 6.5 months to 7 years. All the statistically processed results are obtained from a number of animals which would secure 95% reliability and accuracy of the results, which would allow for sufficient generalization. Differences concerning the influence of age were not demonstrated in any of the determined biochemical values. The results are regarded as representative standards which can be used for clinical and laboratory diagnostics and prognostics in veterinary cynology and for clinical physiology of the German Shepherd dog breed.
 ...
PMID:[Selected biochemical values of clinically healthy dogs]. 677 70

A multi-enzyme reference material was prepared from seven enzymes of asparatate aminotransferase (AST, EC 2.6.1.1), alanine aminotransferase (ALT, EC 2.6.1.2), alkaline phosphatase (ALP, EC 3.1.3.1), lactate dehydrogenase (LD, EC 1.1.1.27), creatine kinase (CK, EC 2.7.2.2), gamma-glutamyltranspeptidase (gamma-GT, EC 2.3.2.2) and amylase (AMY, EC 3.2.1.1) which were purified from human sources including established human cell lines. The enzymatic properties of the material closely resembled those of human serum. In lyophilized form the preparation was stable for at least 200 days when stored at 40 degrees C. Intermethod comparisons of the enzyme activities in 80 clinical specimens were done by correcting the mean values with calibration constants for different assay methods resulting from use of a human serum, the multi-enzyme reference and a commercial control serum. The results from the comparison for the six enzymes of AST, ALT, LD, CK, gamma-GT and AMY in use of the multi-enzyme reference were almost the same as those with use of a human serum as a calibrator, but were not satisfactory for ALP. Even though further search for more reliable material for ALP is required the multi-enzyme reference material can be used for standardization in clinical chemistry.
 ...
PMID:Multi-enzyme reference material from established human cell lines and human sources. 753 22

We have developed a new multienzyme control serum, Seraclear-HE, which was designed to function not only as an accuracy and precision control serum but also as an intermethod calibrator for unifying interlaboratory clinical enzyme data in terms of reference method values. Seraclear-HE contains as analytes the following enzymes of human origin only: aspartate aminotransferase (AST, EC 2.6.1.1) and lactate dehydrogenase (LD, EC 1.1.1.27) from erythrocytes; alanine aminotransferase (ALT, EC 2.6.1.2) from a hepatoma cell line; alkaline phosphatase (ALP, EC 3.1.3.1) from an amnion cell line; creatine kinase (CK, EC 2.7.3.2) from an embryo kidney cell line; gamma-glutamyltransferase (GGT, EC 2.3.2.2) from a macrophage cell line; and amylase (AMY, EC 3.2.1.1) from urine and saliva. The seven partly purified enzymes were lyophilized in partially delipidated human serum containing sucrose (50 g/L), pyridoxal 5'-phosphate (30 mmol/L), and other stabilizers. The material is stable for at least 2 years at temperatures < or = 10 degrees C. For two concentrations of this preparation, reference method values (mainly International Federation of Clinical Chemistry and Japan Society of Clinical Chemistry) obtained at both 30 degrees C and 37 degrees C are assigned.
 ...
PMID:Multienzyme control serum (Seraclear-HE) containing human enzymes from established cell lines and other sources. 1: Preparation and properties. 753 43

PD 138142-15 is a substituted urea hypolipidemic and potential anti-atherosclerotic agent. To determine the toxicity of PD 138142-15, beagle dogs were given oral doses of 1, 10, 30, and 100 mg/kg daily for 13 weeks. Two animals at 100 mg/kg were euthanized during Week 5 due to poor condition. Clinical findings included decreased serum albumin at > or = 30 mg/kg, and increased ALP (up to 30-fold) and 5'-nucleotidase activities (up to 9-fold) at doses > or = 10 mg/kg. ALT and AST activities were elevated only at 100 mg/kg. There was a two- to threefold increase in cytochrome P450 content of hepatic microsomes from all treated animals and increases in liver weights at 10 mg/kg and above. Hepatic changes included hepatocellular hypertrophy and increased cytoplasmic eosinophilia at > or = 10 mg/kg; single cell necrosis of hepatocytes was noted in moribund animals. ACTH-stimulated cortisol levels were decreased at 30 and 100 mg/kg. Adrenal cholesterol esters were decreased at 10 mg/kg and above, while total adrenal cholesterol was decreased at > or = 30 mg/kg. These changes correlated with adrenal cortical zonal atrophy, principally of the zona fasciculata and zona reticularis, present at 30 and 100 mg/kg. Plasma concentrations of PD 131842-15 increased with increasing dose; plasma levels were significantly lower during Week 12 than those on Day 1, possibly due to autoinduction. Overt hepatotoxicity occurred at 100 mg/kg, whereas hepatic changes at 10 and 30 mg/kg were consistent with cytochrome P450 induction. The hepatic lesions were reversible within 4 weeks, while adrenal lesions were still evident after 4 weeks without treatment.
 ...
PMID:Hepatic and adrenal toxicity of a novel lipid regulator in beagle dogs. 758 16

The authors report a study in which they evaluate the efficacy of some laboratory parameters for monitoring intrasplenic hepatocyte xenotransplantation (mouse to rat) as an alternative to 99Tc-HIDA dynamic scan and histologic exam. Swiss mouse and wistar rat hepatocytes were obtained with collagenase digestion. Wistar male rats were used as recipient and were allocated into three groups: A) omotransplanted rats; B) xenotransplanted rats; C) xenotransplanted and immunosuppressed (Cyclosporin A: 20 mg/kg/daily orally) rats. All rats underwent > 70% hepatectomy. Blood samples were obtained daily from a femoral vein and AST, ALT, ALP, bilirubin, albumin and urea were measured. No statistical differences were observed among groups and the laboratory parameters tested can't be considered a valid technique to xenotransplant rejection monitoring.
 ...
PMID:[Monitoring of hepatocyte xenotransplantation. Usefulness of various laboratory parameters]. 761 63

In this study we observed the discriminative ability of five commonly measured laboratory tests to distinguish between gallstone- and non-gallstone-associated pancreatitis. We also assessed the ability of the lipase-amylase ratio to discriminate between alcohol- and non-alcohol-induced pancreatitis. One hundred sixty-two patients with acute pancreatitis were included in the study. Group A consisted of patients presenting to our hospital in 1988 and 1989. Group B consisted of patients presenting in 1992. Models developed using group A patients were validated using group B patients. For gallstone pancreatitis, AST (threshold value 80 IU/liter) alone and a three-factor model, AST, ALP and bilirubin (threshold values of 80 IU/liter, 115 IU/liter, and 15 mumol/liter, respectively) were the best predictors, correctly classifying at least 80% of cases in group A and B. A lipase-amylase ratio of two correctly classified only 48% of cases in group A and 54% in group B. We conclude that biochemical models are useful in predicting the presence of gallstone pancreatitis but not alcoholic pancreatitis.
 ...
PMID:Biochemical models as early predictors of the etiology of acute pancreatitis. 768 46

The objective of the paper was to test efficiency of feed ration enriched with calcium, phosphorus salts and fat concentrate for elimination of negative impacts of magnesite light ashes in beef bulls kept in an exposure area of magnesite works. For this purpose, 24 animals received a feed mix fortified with dicalcium phosphate at a rate of 100 g per head/day for eight months (P1 group) and another group of 24 bulls were administered a feed mix with an addition of 8% corn fat concentrate (P2 group). The other 24 animals were control (control group K). The clinical picture involved the occurrence of mild to profuse diarrheas which were alternately characteristic of all animal species in the first two months of the trial. Feed intake of the investigated groups was equal. Supplementation of feed ration with dicalcium phosphate and corn concentrate increased the weight gains of experimental animals in comparison with control bulls, the increase being 13.2 and 24.5%, resp. In comparison with the control bulls, the intake of the above supplements did not basically influence the dynamics of hematological profile indicators in the experimental bulls (Figs. 1-4). As for the parameters of hepatic profile, in the 3rd month of testing AST activity was positively influenced in both experimental groups if compared with the control group (P < 0.01), Fig. 5, and at the end of observation ALT activity in P2 group (P < 0.01), Fig. 6. Bilirubinemia dynamics did not change in the investigated groups after administration of either supplement (Fig. 9). ALP activity maintained statistically insignificantly higher values in the control animals in the second half of the trial, which demonstrated impairment of mineral metabolism in this group (Fig. 8). Significant differences in IgC levels between the control and experimental groups were confirmed in the 3rd month of the trial (P < 0.01), Fig. 12. In comparison with the control animals, the effect of dietary dicalcium phosphate supplementation in the experimental group P1 and dietary fat extract supplementation in the P2 group was observed in Ca, P and Mg concentrations to a more significant extent in the examined organs as well as in blood serum (Tabs. I-V). Except in spleen, there was a trend of higher cumulation of Ca in all the examined organs of bulls receiving dicalcium phosphate supplement. Phosphorus cumulation showed the same dependence upon dicalcium phosphate intake in the examined organs. Mg deposition in all examined organs showed minimum differences between the experimental groups and control animals.(ABSTRACT TRUNCATED AT 400 WORDS)
 ...
PMID:[Possibilities of eliminating the effect of magnesite fly ash in beef bulls]. 774 Jul 14

Fasting bile acid, two-hour post prandial bile acid and other liver function tests (Bili, AST, ALT, ALB, Glob, ALP) were measured in 22 normal and 28 liver diseased patients. In normal volunteers, the mean value of fasting total serum bile acid (FTBA) and postprandial serum bile acid (PTBA) were 3.08 mumole/L (S.D. 1.65) range 0.21-6.26 mumol/L, and 8.07 mumole/L (S.D. 2.99) range 4.06-15.65 mumole/L. Comparison between FTBA, PTBA and other liver function tests in various liver diseases from this study the PTBA was not statistically significant superior to FTBA. Therefore, it is not necessary to do the PTBA at this time until more data is available.
 ...
PMID:Comparison study between fasting total serum bile acid and post prandial bile acid in hepatic diseases: a preliminary study. 779 28


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>