Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:2.6.1.1 (aspartate aminotransferase)
 21,665 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An original method for the collection of pure or nearly pure pulmonary lymph in the canine is desirable for the study of pulmonary water, protein dynamics and cells. Right lymphatic duct lymph has been used extensively but it known to contain lymph from a number of extrapulmonary sources and has been altered by passage through lymph nodes. Pulmonary lymph was collected from 13 dogs through an open chest. The mean flow of lymph was 1.5 milliliters per hour +/- 0.08. This flow is compared with 3.7 milliliters per hour from the right lymphatic duct and 22.5 milliliters per hour from the thoracic duct in a group of dogs with a closed chest. The levels of lactate dehydrogenase--634 units per liter--and glutamic-oxalacetic transaminase--416 units per liter--in pure pulmonary lymph were much higher than in right lymphatic duct lymph--lactate dehydrogenase 125 units per liter; glutamic-oxalacetic transaminase 94.0 units per liter, in thoracic duct lymph--lactate dehydrogenase 47 units per liter; glutamic-oxalacetic transaminase 80.5 units per liter--and in blood plasma--lactate dehydrogenase 299 units per liter; glutamic-oxalacetic transaminase 95 units per liter. Low levels were noted in Na+, 107, and Cl-, 85, in pure pulmonary lymph versus plasma--Na+ 145; Cl- 112, right lymphatic duct lymph--Na+ 146; Cl- 115, and thoracic duct lymph--Na+ 146; Cl- 114. The method can be adapted for prolonged drainage in conscious dogs, which would enhance its usefulness.
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PMID:A method for the collection of pure pulmonary lymph in the canine. 710 Nov 13

Aspartate aminotransferase (EC 2.6.1.1:AST) is known to have two isoenzymes, one associated with the cytoplasm (c-AST) and the other with the mitochondria (m-AST). We studied the relationships of m-AST activity in the coronary sinus blood to left ventricular function, coronary blood flow, water content and high-energy phosphate stores of the left ventricle following hypothermic ischemic cardiac arrest. Under cardiopulmonary bypass with hypothermia of 20 degrees C of myocardial temperature, 120 min of aortic occlusion was employed in 15 mongrel dogs. Left ventricular function (peak left ventricular pressure, left ventricular end-diastolic pressure, max dp/dt, cardiac index, left ventricular stroke work index), coronary blood flow, myocardial oxygen consumption, myocardial enzyme activity (m-AST, CK-MB), myocardial water content and high-energy phosphate stores (adenosine triphosphate, creatine phosphate) of the subendocardium of the left ventricle were measured. Data was obtained in the control state, and after 0, 30 and 60 min of reperfusion. Significant negative correlations were obtained between m-AST activity and peak left ventricular pressure (r = -0.81, p less than 0.001), max dp/dt (r = -0.83, p less than 0.001), cardiac product (r = -0.73, p less than 0.01), coronary blood flow (r = -0.59, p less than 0.05), adenosine triphosphate level (r = 0.72, p less than 0.01) and creatine phosphate level (r = -0.72, p less than 0.02) after 60 min of reperfusion. Significant positive correlations were obtained between m-AST activity and left ventricular end-diastolic pressure (r=0.75, p less than 0.01) and water content (r = 0.78, p less than 0.01) after 60 min of reperfusion. These results led to the assumption that serum m-AST activity in the coronary venous blood is a useful index to evaluate the degree of myocardial injury.
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PMID:Studies on the significance of serum mitochondrial aspartate aminotransferase activity following ischemic cardiac arrest. 714 3

1. The effects of implanting turkeys with trienbolone acetate (TA) upon fluid balance and blood chemistry were studied. 2. The Na and water contents of skeletal muscles were increased by TA treatment while K was unaltered. 3. The extracellular space expressed as a proportion of starved body weight was unaffected by TA implantation. 4. Plasma or serum concentrations of P, Ca, Mg, Na and K and activities of the enzymes aspartate aminotransferase [EG 2.61.1], creatine kinase [EC 2.7.3.2] and gamma-glutamyl transferase [EC 2.3.2.2] were not changed by TA treatment. 5. Packed cell volume was significantly increased by TA implantation after a delay of some 2 to 3 weeks while plasma protein concentrations were immediately decreased for a period of two weeks before nearly normal concentrations were obtained again. 6. Erythrocyte sedimentation rate was decreased by TA treatment, but serum protein electrophoretic pattern was unchanged.
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PMID:The effects of trienbolone acetate implantation of turkeys upon fluid balance and blood chemistry. 726 Jul 1

Exposure to simulated weightlessness (7-day water immersion and 7-day head-down tilt) caused a decrease in the activity of malate (MDH) and isocitrate dehydrogenase (ICDH), and creatine phosphokinase dehydrogenase (ICDH), and creatine phosphokinase (CPK) at the expense of its MM isoform whereas the activity of alanine (ALT) and aspartate aminotransferase (AST) and pattern of distribution of MDH isoforms remained unchanged. Exposure to acceleration of +3 Gz before and after simulated weightlessness revealed similar changes in the activity of MDH, ICDH, ALT, AST and MDH cytoplasmic fractions. However, the higher increase in the enzyme activity after simulated weightlessness may give evidence for a greater change in cell membrane permeability during acceleration effects that followed simulated weightlessness.
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PMID:[Energy-metabolism enzymes during combined exposure of the body to simulated weightlessness and gravitational overloads]. 728 61

Diisopropyl methylphosphonate (DIMP), and dicyclopentadiene [3a,4,7,7a-tetrahydro-4,7-methyanoindene] (DCPD), were found as contaminants of groundwater in Colorado. Since there was a potential for cattle to be exposed to these chemicals by drinking well water, a study of their effects was initiated. Eight-to-ten week old calves were given a single dose of either DIMP at 62.5, 125, 250, 500 and 1000 mg/kg of body weight (b.w.) or DCPD at 250, 500, 1000 or 2000 mg/kg of b.w. The calves given DIMP developed tympanitis and ataxia, followed by depression, prostration, and death within two hr after dosing. A slight but significant increase in activated partial thromboplastin time was the only change observed in any of the clinical pathologic parameters. The only gross pathologic changes were acute gastroenteritis with hemorrhages in calves given 1000 mg/kg of b.w. Mild signs of intoxication, ataxia and excess salivation, were observed in calves given 250 mg of DCPD/kg of b.w. At higher doses, these signs were intensified; in addition, calves fell and, while prostrate, exhibited running movements and tonic, clonic spasms. The severity of the signs observed increased as the dose of DCPD increased. All calves given 2000 mg/kg of b.w. and one calf given 1000 mg/kg of b.w. died before seven days after dosing. The only clinical pathologic changes found were increased serum levels of creating phosphokinase, glutamic-oxalacetic transaminase, and glutamic pyruvic transaminase. The only consistent gross pathologic change was congestion in a variety of tissues in calves given 2000 mg/kg of b.w. A variety of histologic changes were observed in tissues from calves treated with both chemicals. However, these changes were not consistent for any one dose level and were not dose dependent. DIMP was slightly toxic for calves, since no signs of intoxication were observed at doses less than 1000 mg/kg of b.w. DCPD exerted detrimental effects on calves at 250 mg/kg of b.w. and was classified as moderately toxic.
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PMID:Toxicologic evaluation of diisopropyl methylphosphonate and dicyclopentadiene in cattle. 730 51

Acute and chronic effects of Se as sodium selenite given as a supplement in the drinking water of Sprague-Dawley rats for 35 d, 1 yr, and 2 yr are compared. For the 35-d study the experimental groups were untreated controls and rats supplemented with 1, 4, 8, 16, and 64 ppm Se. Survival was 100% in the control and 1 and 4 ppm groups, decreased in the 8 and 16 ppm groups, and was zero in the 64 ppm group. Body weights increased and were equivalent in the control and 1 and 4 ppm groups and substantially decreased in the 16 and 64 ppm groups., Serum alkaline phosphatase and glutamic-oxaloacetic transaminase (SGOT) increased with 16 ppm Se and higher supplements. Se toxicity was apparent in microscopic pathology showing liver congestion, fatty degeneration of parenchymal cells, and necrosis. In the chronic studies untreated controls are compared with rate receiving 4 ppm Se in the drinking water. In general, the weight gains throughout were equivalent for both groups. The 1-yr survival in each was above 90% and the 2-yr survival above 50%. With increased age there was a slight reduction in hemoglobin and white blood cells. The latter effect was greater in Se-treated then in control rats. Several serum components were equivalent in both groups, including alkaline and acid phosphatase, SGOT, protein, glucose, and sialic acid. Liver glutathione peroxidase was half and Se levels in the Se-treated rats were twice those in the controls. Data are presented for male rats in the chronic study with occasional reference to data on females. The parameters measured in the chronic study are highly dependent on the age of the rat when Se-supplemented drinking water is initiated.
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PMID:Toxicological effects of sodium selenite in Sprague-Dawley rats. 733 30

Se as sodium selenite was administered by gavage (three consecutive times) and as drinking water supplements for 46 d to male and female Swiss mice. With respect to survival in 7-wk-old mice, Se was less toxic in males than in females when gavaged. Drinking water supplements of 1-64 ppm Se resulted in 1 male and 1 female death in mice first given Se at 7 wk of age. Se supplements to the drinking water of adult (18-wk-old) mice was less toxic in females. All young (7-wk-old) and adult (18-wk-old) mice provided 1-16 ppm Se in the drinking water survived the 46-d treatment, but in adult mice 64 ppm Se significantly reduced survival. Only 64 ppm Se supplements caused a sharp reduction in body weight in young and adult mice of both sexes. Supplements of 1-8 ppm Se in all mice elicited growth responses similar to those of untreated controls. Occasional liver and kidney congestion, liver necrosis, parenchymal cell degeneration, and bile duct proliferation were observed in control and treatment groups. Serum alkaline phosphatase and glutamic-oxaloacetic transaminase (SGOT) increased with 32 ppm Se and higher supplements. Survival, growth, serum enzymes, and pathology were normal in untreated controls and in mice of growth ages and sexes give 1, 4, and 8 ppm Se supplements. A chronic toxicity study was conducted in female Swiss mice given 1, 4, and 8 ppm Se supplements for 50 wk. The survival of Se-treated groups was more than 90% and that of controls was only 72% after 50 wk. All mice gained weight, but the group treated with 8 ppm Se gained half as much as other groups. Both liver Se and glutathione peroxidase activity increased in Se-treated mice compared to controls at 25 and 50 wk. A reduced white blood cell count and increased alkaline phosphatase and SGOT suggested a mild toxic effect of the 8 ppm Se supplement in the chronic study.
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PMID:Toxicological effects of sodium selenite in Swiss mice. 733 31

Some analytical parameters have been investigated for a recently described stabilized liquid coenzyme technology in which water-free NAD is dissolved in 1,2 propanediol. Correlation for 108 specimens assayed for AST, ALT and LD with a reference method in which glycol-based NAD was absent was greater than or equal to 0.998 with near identical reproducibility over a period of at least 107 days. Mean recovery of exogenous serum enzymes in this linear kinetic assay is 103%. With the option of mixing only the volume of reagent needed for the enzymatic assay, waste can be eliminated as compared to more costly preparations stabilized by lyophilization. Hazards from an impure water supply are avoided since no reconstituting volume is required.
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PMID:Utilization of a glycol-stabilized liquid NAD for the measurement of three enzymes on the GEMSAEC. 736 51

Conditions were determined for rapid separation of cytosolic and mitochondrial compartments by digitonin fractionation of rat hepatocytes. The minimum time required for separation of mitochondrial and cytosolic enzyme markers decreased rapidly with increasing temperature. Kyro EOB, a non-ionic detergent, increases the release of cytosolic enzymes, particularly at lower temperatures. Experimental procedures are described for greater than 90% release of cytosolic enzymes and less than 2% release of mitochondrial enzymes in 3s. By using appropriate concentrations of digitonin and Kyro EOB in a fractionation medium maintained at 1 degrees C and a minimum time of exposure to the medium, nearly separate patterns of release were obtained for enzyme markers for the cytosol, mitochondrial matrix and mitochondrial intermembrane space. The distribution of enzymes that exist in more than one of these compartments was quantified by comparing their rates of release with those of marker enzymes. The cytosol/mitochondrial-matrix distributions for such enzymes in hepatocytes from starved rats were 16%/84% for aspartate aminotransferase, 34%/66% for fumarase and 77%/23% for ATP citrate lyase. In hepatocytes from rats that were induced to synthesize ATP citrate lyase by starvation and re-feeding, the ratio had increased to 95%/5%. The maximum cytosol/intermembrane-space ratio for adenylate kinase was 8%/92%. A procedure is also described for treating commercial digitonin that increases its solubility in water from about 1mg/ml to more than 800mg/ml.
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PMID:Subcellular distribution of enzymes determined by rapid digitonin fractionation of isolated hepatocytes. 737 59

Activities of glutamic-oxalacetic transaminase, glutamate dehydrogenase, gamma-glutamyl transferase were reduced in unstimulated pooled saliva of 15-year-old adolescents with endemic fluorosis, vs. those without fluorosis. This point to reduced production of glutamate which is indispensable for bacterial growth. The activity of acid phosphatase in pooled stimulated with 1% pilocarpin saliva of rats fed a water ration with 5 and 20 mg/liter fluorine in comparison with control rats fed water with fluorine concentration of 0.21 mg/liter. Noteworthy that alkaline phosphatase activity was virtually unchanged at fluorine concentrations 5 and 20 mg/liter in the water. The significance of the results as far as it regards the pathogenesis of caries and fluorosis is discussed.
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PMID:[The enzymes of glutamate and organic phosphate metabolism in the saliva in fluorosis (clinical and experimental research)]. 748 2


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