Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:2.5.1.18 (glutathione S-transferase)
 22,582 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

MAGOH is the human homologue of Drosophila mago nashi, a protein that is required for normal germ plasm development in the Drosophila embryo. Using human MAGOH as a bait protein in a yeast two-hybrid screen, we recovered four independent cDNA clones that encode different lengths of a novel protein containing a conserved RNA-binding region. This gene, designated RBM8, encodes a 173-aa protein that was shown to have an apparent molecular mass of 26 kDa, as demonstrated by in vitro translation assay. The interaction between MAGOH and RBM8 was demonstrated by both yeast two-hybrid and GST fusion protein pull-down assays. Like MAGOH, RBM8 gene is expressed ubiquitously in human tissues; three species of RBM8 mRNA were detected. Also similar to MAGOH, RBM8 expression is serum inducible in quiescent NIH3T3 fibroblast cells.
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PMID:MAGOH interacts with a novel RNA-binding protein. 1066 55

Mago nashi (Mago) and Y14 proteins are highly conserved among eukaryotes, and not only play important roles in oogenesis, embryogenesis and germ-line determination during animal development, but also participate in mRNA localization and splicing in cell growth. We identified mago (Tcmago) and Y14 (TcY14) homologues derived from expressed sequence tags of Taiwania cryptomerioides Hayata seedlings. Tcmago and TcY14 contain coding regions encoding 149 and 216 amino acids, respectively. Multiple amino acid sequence alignments as well as secondary and tertiary structure all predicted that TcMago and TcY14 possessed similar protein structures to the crystal structures of Drosophila melanogaster and human Mago and Y14 proteins. We demonstrated by yeast two-hybrid analysis and a GST pull-down assay that TcMago and TcY14 interacted in vivo and in vitro, confirming structural predictions of their interaction. TcMago and TcY14 were predominately localized in the nucleus. Whole mount in situ hybridization and immunolocalization showed that Tcmago and TcY14 were both detected in root hairs at the levels of transcription and protein expression. Overexpression of Tcmago in transgenic tobacco plants resulted in longer roots and a more complex root system. TcMago and TcY14 may have cellular functions similar to the Mago and Y14 proteins in animals and may be involved in root development in plants.
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PMID:Molecular identification and characterization of Tcmago and TcY14 in Taiwania (Taiwania cryptomerioides). 1754 26