Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.5.1.18 (
glutathione S-transferase
)
22,582
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We have cloned and characterized mouse and human variants of
MONaKA
, a novel protein that interacts with and modulates the plasma membrane Na,K-ATPase.
MONaKA
was cloned based on its sequence homology to the Drosophila Slowpoke channel-binding protein dSlob, but mouse and human
MONaKA
do not bind to mammalian Slowpoke channels. At least two splice variants of
MONaKA
exist; the splicing is conserved perfectly between mouse and human, suggesting that it serves some important function. Both splice variants of
MONaKA
are expressed widely throughout the CNS and peripheral nervous system, with different splice variant expression ratios in neurons and glia. A yeast two-hybrid screen with
MONaKA
as bait revealed that it binds tightly to the beta1 and beta3 subunits of the Na,K-ATPase. The association between
MONaKA
and Na,K-ATPase beta subunits was confirmed further by coimmunoprecipitation from transfected cells, mouse brain, and cultured mouse astrocytes. A
glutathione S-transferase
-
MONaKA
fusion protein inhibits Na,K-ATPase activity from whole brain or cultured astrocytes. Furthermore, transfection of
MONaKA
inhibits 86Rb+ uptake via the Na,K-ATPase in intact cells. These results are consistent with the hypothesis that
MONaKA
modulates brain Na,K-ATPase and may thereby participate in the regulation of electrical excitability and synaptic transmission.
...
PMID:MONaKA, a novel modulator of the plasma membrane Na,K-ATPase. 1613 50
