Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.5.1.18 (
glutathione S-transferase
)
22,582
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Melanoma is the most aggressive and lethal type of skin cancer. The aim of the present study was to illustrate the molecular mechanism of
makorin ring finger protein 2
(
MKRN2
) control of melanoma cell proliferation. The expression level of
MKRN2
was detected in human malignant melanoma cell lines by immunoblotting and reverse transcription-quantitative PCR. Short hairpin RNAs for
MKRN2
were designed and transfected into melanoma cells, and the proliferation of these cells was detected by MTT and colony formation assays. The interaction of
MKRN2
with P53 was detected by co-immunoprecipitation and
glutathione S-transferase
pulldown assays. The ubiquitination of P53 by
MKRN2
was detected by
in vitro
ubiquitination assays. A P53-knockout cell line was generated using the CRISPR-Cas9 method.
MKRN2
exhibited higher expression levels in melanoma cells, and downregulation of
MKRN2
inhibited melanoma cell growth in a P53-dependent manner.
MKRN2
regulated melanoma cell proliferation by interacting and ubiquitylating P53, which suggests that
MKRN2
may be a potential therapeutic target for melanoma.
...
PMID:Ubiquitination of P53 by E3 ligase MKRN2 promotes melanoma cell proliferation. 3219 92
