Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.5.1.18 (
glutathione S-transferase
)
22,582
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
To explore more possible roles for GSK3beta function, the yeast two-hybrid screening using GSK3beta as a bait protein was performed. In this study, we demonstrated that two variants of
CABYR
(281 and 379) interacted with GSK3beta in the yeast two-hybrid and
GST
pull down assay. Molecular characterization showed that
CABYR
variants formed a dimer with a proline-rich extensin-like domain, which slightly overlapped with GSK3beta-binding site. In kinase assay, we also showed that
CABYR
variants act as an ideal substrate for GSK3beta within the extensin-like domain and phosphorylation sites on
CABYR
were mapped. Interestingly, Northern blot showed that
CABYR
transcripts were expressed more distinctly in the fetal brain than in the adult brain, suggesting that this protein may play a role during brain development. Moreover, differential expression of
CABYR
variants may exhibit aberrant expression in brain tumors and cancer cell lines.
...
PMID:Characterization of two non-testis-specific CABYR variants that bind to GSK3beta with a proline-rich extensin-like domain. 1575 68
