Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.5.1.18 (
glutathione S-transferase
)
22,582
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The eukaryotic lipid kinase phosphatidylinositol 4-kinase III alpha (
PI4KA
in higher eukaryotes) is a ubiquitous enzyme that synthesizes the plasma membrane pool of phosphatidylinositol 4-phosphate. This important phosphoinositide has key roles in different signalization pathways, vesicular traffic and cellular compartment identity. Moreover, human PI4K4A is an essential factor for hepatitis C virus replication.
PI4KA
is a large protein (2102 residues for human
PI4KA
) with the kinase domain making up the ca 400 C-terminal residues. There is essentially no structural information about the 1500N-terminal residues and no clue as to the function of most of this region of
PI4KA
. In this report, we use computational methods in order to delineate fragments of human
PI4KA
amenable to soluble production in Escherichia coli. We clone and express these fragments as
GST
-fusions and evaluate the soluble fraction of each protein. Finally, we produce and purify to homogeneity a 1100-residue
PI4KA
N-terminal fragment. Our results further suggest that
PI4KA
can be described as a two-module protein. They open the way to structural characterization of the N-terminal regulatory module of
PI4KA
.
...
PMID:Definition and expression in E. coli of large fragments from the human lipid kinase phosphatidylinositol 4-kinase type III alpha, and purification of a 1100-residue N-terminal module. 2614 34
