Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.5.1.18 (
glutathione S-transferase
)
22,582
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The pGEX system for protein production in E. coli is widely used in molecular biology. A bacterial expression vector, pETGEXCT, which incorporates features of the pGEX and pET expression systems was designed. pETGEXCT allows the production of N- and C-terminal fusions to
glutathione S-transferase
(
GST
) under the tight control of the T7 promoter. Use of this vector can circumvent problems associated with unstable or inactive fusions to the N terminus of
GST
. Indeed, it is demonstrated that fusions to the N terminus of the eukaryotic DNA-binding protein,
RSRFC4
, cannot be tolerated. Fusion of
RSRFC4
to the N terminus of
GST
in the pETGEXCT vector is a prerequisite to purify the
RSRFC4
DNA-binding domain in an active form using glutathione-agarose affinity chromatography.
...
PMID:A T7 expression vector for producing N- and C-terminal fusion proteins with glutathione S-transferase. 812 85
