Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.5.1.18 (
glutathione S-transferase
)
22,582
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A recombinant gene CCS consisting of three single genes (CAP-ScFv,
CPFX
-ScFv and SM
2
-ScFv) were constructed by PCR with three pairs of primers. The length of CCS gene fragment was 2,260 bp. A recombinant plasmid (pGEX-CCS) was obtained in pGEX-6p-1. pGEX-CCS was induced in
E.coli
BL21(DE3), and the molecular weight of recombinant fusion protein (
GST
-CCS) was 108.87 kDa.
GST
-CCS was successfully applied to analysis of CAP-OVA (CAP-ovalbumin conjugate),
CPFX
-OVA and SM
2
-OVA simultaneously. The sensitivity of
GST
-CCS against three veterinary drugs was tested by indirect ELISA at dilution ratio 1:8000. These findings provide a foundation for the construction of fusion genes with linkers and for the potential development of a rapid screening method for the simultaneous detection of veterinary drug residues.
...
PMID:An exploratory study on the simultaneous screening for residues of chloramphenicol, ciprofloxacin and sulphadimidine using recombinant antibodies. 3210 97
