Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:2.5.1.18 (
glutathione S-transferase
)
22,582
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
HA95
is a chromatin-associated protein that interfaces the nuclear envelope (NE) and chromatin. We report an interaction between
HA95
and the inner nuclear membrane protein lamina-associated polypeptide (LAP) 2 beta, and a role of this association in initiation of DNA replication. Precipitation of
GST
-LAP2 beta fusion proteins and overlays of immobilized
HA95
indicate that a first
HA95
-binding region lies within amino acids 137-242 of LAP2 beta. A second domain sufficient to bind
HA95
colocalizes with the lamin B-binding domain of LAP2beta at residues 299-373.
HA95
-LAP2 beta interaction is not required for NE formation. However, disruption of the association of
HA95
with the NH2-terminal
HA95
-binding domain of LAP2 beta abolishes the initiation, but not elongation, of DNA replication in purified G1 phase nuclei incubated in S-phase extract. Inhibition of replication initiation correlates with proteasome-mediated proteolysis of Cdc6, a component of the prereplication complex. Rescue of Cdc6 degradation with proteasome inhibitors restores replication. We propose that an interaction of LAP2beta, or LAP2 proteins, with
HA95
is involved in the control of initiation of DNA replication.
...
PMID:HA95 and LAP2 beta mediate a novel chromatin-nuclear envelope interaction implicated in initiation of DNA replication. 1253 39
